{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:15:17Z","timestamp":1769750117157,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A variable-number tandem repeat (VNTR) protocol targeting 10 loci in the\n            <jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>\n            genome was used to assess genetic diversity among 366 field isolates recovered from cattle, bison, and elk in the Greater Yellowstone Area (GYA) and Texas during 1998 to 2011. Minimum spanning tree (MST) and unweighted-pair group method with arithmetic mean (UPGMA) analyses of VNTR data identified 237 different VNTR types, among which 14 prominent clusters of isolates could be identified. Cattle isolates from Texas segregated into three clusters: one comprised of field isolates from 1998 to 2005, one comprised of vaccination-associated infections, and one associated with an outbreak in Starr County in January 2011. An isolate obtained from a feral sow trapped on property adjacent to the Starr County herd in May 2011 clustered with the cattle isolates, suggesting a role for feral swine as\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            reservoirs in Starr County. Isolates from a 2005 cattle outbreak in Wyoming displayed VNTR-10 profiles matching those of strains recovered from Wyoming and Idaho elk. Additionally, isolates associated with cattle outbreaks in Idaho in 2002, Montana in 2008 and 2011, and Wyoming in 2010 primarily clustered with isolates recovered from GYA elk. This study indicates that elk play a predominant role in the transmission of\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            to cattle located in the GYA.\n          <\/jats:p>","DOI":"10.1128\/aem.00045-12","type":"journal-article","created":{"date-parts":[[2012,3,17]],"date-time":"2012-03-17T03:41:44Z","timestamp":1331955704000},"page":"3674-3684","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Molecular Epidemiology of Brucella abortus Isolates from Cattle, Elk, and Bison in the United States, 1998 to 2011"],"prefix":"10.1128","volume":"78","author":[{"given":"James","family":"Higgins","sequence":"first","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tod","family":"Stuber","sequence":"additional","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Quance","sequence":"additional","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Wyoming Game and Fish Department, Laramie, Wyoming, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebekah V.","family":"Tiller","sequence":"additional","affiliation":[{"name":"U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Linfield","sequence":"additional","affiliation":[{"name":"USDA-APHIS Veterinary Services, Helena, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Rhyan","sequence":"additional","affiliation":[{"name":"USDA-APHIS Veterinary Services, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Berte","sequence":"additional","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth","family":"Harris","sequence":"additional","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"AltonGG\nJonesLM\nAngusRD\nVergerJM. 1988. Techniques for the brucellosis laboratory p 34\u201361. INRA Publications Paris France."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-45.4.1174"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg491"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870301500413"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-3-15"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-37"},{"key":"e_1_3_3_8_2","unstructured":"ChevilleNF\nMcCulloughDR. 1998. Brucellosis in the Greater Yellowstone Area p 16\u201388. National Research Council Press Washington DC."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1890\/06-1603"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1890\/08-2062.1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-15.3.367"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01494-07"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-42.2.271"},{"key":"e_1_3_3_14_2","first-page":"97","article-title":"Identification and differentiation of Brucella abortus field and vaccine strains by BASS-PCR","volume":"216","author":"Ewalt DR","year":"2003","unstructured":"EwaltDR BrickerBJ. 2003. Identification and differentiation of Brucella abortus field and vaccine strains by BASS-PCR. Methods Mol. Biol. 216:97\u2013108.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-569-5_6"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02538-10"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.03.051"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-9"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-31.4.579"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/89.3.583"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/elr026"},{"key":"e_1_3_3_22_2","first-page":"729","volume-title":"J. Wildl. Dis.","author":"Rhyan JC","year":"2009","unstructured":"RhyanJC. 2009. Pathogenesis and epidemiology of brucellosis in Yellowstone bison: serologic and culture results from adult females and their progeny. J. Wildl. Dis. 45:729\u2013739."},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2307\/3802831"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-46.2.442"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.51.5.873-884.1986"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870701900301"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02362-08"},{"key":"e_1_3_3_28_2","unstructured":"USDA-APHIS. 2011. National brucellosis slaughter surveillance plan. USDA-APHIS Washington DC. http:\/\/www.aphis.usda.gov\/animal_health\/animal_diseases\/brucellosis\/downloads\/nat_bruc_slaughter_surv_plan.pdf."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02039-05"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-34"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00045-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00045-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:27:07Z","timestamp":1645644427000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00045-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,15]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2012,5,15]]}},"alternative-id":["10.1128\/AEM.00045-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.00045-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,15]]},"assertion":[{"value":"2012-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}