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Biopreservatives can be used for the prevention and control of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              in food. In this study, we identified a novel lantibiotic, mycoidesin, with potent bacteriostatic activity against\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              . It exhibited 4- to 16-fold higher bacteriostatic activity against the\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              strains than nisin A. Analysis of the mode of action of mycoidesin revealed that it exerted bacteriostatic activity against\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              ATCC 19111 at low and high concentrations (1\u00d7\u201332\u00d7 MIC, 0.39\u201312.5 \u00b5M). It blocked cell wall synthesis by binding to Lipid II and inhibiting the growth of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              . For other sensitive strains, such as\n              <jats:italic>Bacillus cereus<\/jats:italic>\n              CMCC 63301, mycoidesin exerted a bacteriostatic effect at a low concentration (1\u00d7 MIC, 1.56 \u00b5M) via the same mechanism, whereas it exerted a bactericidal effect at high concentrations (2\u00d7\u20138\u00d7 MIC, 3.13\u201312.5 \u00b5M), which can damage the cell membrane and cause cell death. The stability test showed that mycoidesin had increased stability compared to nisin A. Additionally, mycoidesin showed low cytotoxic and hemolytic activity. Furthermore, mycoidesin effectively inhibited the growth of\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              in beef and delayed the decline in beef quality. Our study demonstrates the potential of mycoidesin as a biopreservative to prevent\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              contamination and improve the safety of meat and meat products in the food industry.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study aimed to identify highly effective, stable, and safe natural bacteriocin preservatives with anti-\n              <jats:italic>Listeria monocytogenes<\/jats:italic>\n              activity. We isolated a novel class II lantibiotic, mycoidesin, which exhibited more efficient bacteriostatic activity against\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              and increased stability compared to the applied bacteriocin food preservative, nisin A. Mycoidesin also showed favorable biosafety. Moreover, mycoidesin could be effectively used for controlling\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              in beef, demonstrating its potential as a biopreservative to prevent\n              <jats:italic>L. monocytogenes<\/jats:italic>\n              -related contamination and improve the safety of meat and meat products in the agricultural and food industries.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.00067-25","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T15:07:16Z","timestamp":1742915236000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Mycoidesin, a novel lantibiotic, exhibits potent bacteriostatic activity against\n            <i>Listeria monocytogenes<\/i>\n            and effectively controls its growth in beef"],"prefix":"10.1128","volume":"91","author":[{"given":"Fei","family":"Zhang","sequence":"first","affiliation":[{"name":"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University","place":["Huaibei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Ding","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University","place":["Huaibei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Liu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University","place":["Huaibei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Huang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulin","family":"Deng","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University","place":["Huaibei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Gao","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University","place":["Huaibei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualin","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Perfect Life Health Science and Technology Research Institute Co. 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