{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:58:39Z","timestamp":1790863119976,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium,\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            A4, was constructed by introducing the biodesulfurizing gene cluster\n            <jats:italic>dszABCD<\/jats:italic>\n            , which was from\n            <jats:italic>Rhodococcus erythropolis<\/jats:italic>\n            XP, into the solvent-tolerant strain\n            <jats:italic>P. putida<\/jats:italic>\n            Idaho. Biphasic reactions were performed to investigate the desulfurization of various sulfur-containing heterocyclic compounds in the presence of various organic solvents.\n            <jats:italic>P. putida<\/jats:italic>\n            A4 had the same substrate range as\n            <jats:italic>R. erythropolis<\/jats:italic>\n            XP and could degrade dibenzothiophene at a specific rate of 1.29 mM g (dry weight) of cells\n            <jats:sup>\u22121<\/jats:sup>\n            h\n            <jats:sup>\u22121<\/jats:sup>\n            for the first 2 h in the presence of 10% (vol\/vol)\n            <jats:italic>p<\/jats:italic>\n            -xylene.\n            <jats:italic>P. putida<\/jats:italic>\n            A4 was also able to degrade dibenzothiophene in the presence of many other organic solvents at a concentration of 10% (vol\/vol). This study is a significant step in the exploration of the biotechnological potential of novel biocatalysts for developing an efficient biodesulfurization process in biphasic reaction mixtures containing toxic organic solvents.\n          <\/jats:p>","DOI":"10.1128\/aem.00081-06","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T01:51:13Z","timestamp":1152237073000},"page":"4604-4609","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Biodesulfurization in Biphasic Systems Containing Organic Solvents"],"prefix":"10.1128","volume":"72","author":[{"given":"Fei","family":"Tao","sequence":"first","affiliation":[{"name":"State Key Lab of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Lab of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Lab of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui Qing","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Lab of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.9.3637-3642.1997"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-3820(03)00007-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1587-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.2723-2729.1992"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(98)01234-7"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20061"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90358-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2000.00151.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.7156-7160.1997"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(03)00065-1"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1296-1705"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1755-7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/338264a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.02.001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007920050065"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.503-549.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie00006a023"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(89)90007-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09147.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09152.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(98)00078-7"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00397-5"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.276-281.2005"},{"key":"e_1_3_2_25_2","unstructured":"Maniatis T. 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