{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:49:02Z","timestamp":1791200942323,"version":"4.2.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The quantification of denitrifying bacteria is a component in the further understanding of denitrification processes in the environment. Real-time PCR primers were designed to target two segments of the denitrifier population (\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>P<\/jats:sub>\n            [\n            <jats:italic>Pseudomonas mandelii<\/jats:italic>\n            and closely related strains] and\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>B<\/jats:sub>\n            [\n            <jats:italic>Bosea<\/jats:italic>\n            ,\n            <jats:italic>Bradyrhizobium<\/jats:italic>\n            , and\n            <jats:italic>Ensifer<\/jats:italic>\n            spp.]) in agricultural soils based on functional\n            <jats:italic>cnorB<\/jats:italic>\n            (nitric oxide reductase) gene sequences. Total population numbers were measured using 16S rRNA gene real-time PCR. Two soil microcosm experiments were conducted. Experiment 1 examined the response of the indigenous soil microbial population to the addition of 500 mg\/kg glucose-C daily over 7 days in soil microcosms. Changes in the total population were correlated (\n            <jats:italic>r<\/jats:italic>\n            = 0.83) between 16S rRNA gene copy numbers and microbial biomass carbon estimates. Members of the\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>P<\/jats:sub>\n            population of denitrifiers showed typical r-strategy by being able to increase their proportion in the total population from starting levels of &lt;0.1% to around 2.4% after a daily addition of 500 mg\/kg glucose-C. The\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>B<\/jats:sub>\n            guild was not able to increase its relative percentage of the total population in response to the addition of glucose-C, instead increasing copy numbers only in proportion with the total population measured by 16S rRNA genes. Experiment 2 measured population dynamics in soil after the addition of various amounts of glucose-C (0 to 500 mg\/kg) and incubation under denitrifying conditions.\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>P<\/jats:sub>\n            populations increased proportionally with the amount of glucose-C added (from 0 to 500 mg\/kg). In soil microcosms, denitrification rates, respiration, and\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>P<\/jats:sub>\n            population densities increased significantly with increasing rates of glucose addition.\n            <jats:italic>cnorB<\/jats:italic>\n            <jats:sub>B<\/jats:sub>\n            guild densities did not increase significantly under denitrifying conditions in response to increasing C additions.\n          <\/jats:p>","DOI":"10.1128\/aem.00081-07","type":"journal-article","created":{"date-parts":[[2007,4,21]],"date-time":"2007-04-21T00:50:32Z","timestamp":1177116632000},"page":"4250-4258","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Nitric Oxide Reductase-Targeted Real-Time PCR Quantification of Denitrifier Populations in Soil"],"prefix":"10.1128","volume":"73","author":[{"given":"C. E.","family":"Dandie","sequence":"first","affiliation":[{"name":"Agriculture and Agri-Food Canada, Potato Research Centre, Fredericton, New Brunswick E3B4Z7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. N.","family":"Miller","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Potato Research Centre, Fredericton, New Brunswick E3B4Z7, Canada"},{"name":"University of Guelph, Department of Environmental Biology, Guelph, Ontario N1G2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. L.","family":"Burton","sequence":"additional","affiliation":[{"name":"Nova Scotia Agricultural College, Department of Environmental Sciences, Truro, Nova Scotia B2N5E3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. J.","family":"Zebarth","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Potato Research Centre, Fredericton, New Brunswick E3B4Z7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. T.","family":"Trevors","sequence":"additional","affiliation":[{"name":"University of Guelph, Department of Environmental Biology, Guelph, Ontario N1G2W1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Goyer","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Potato Research Centre, Fredericton, New Brunswick E3B4Z7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"130","volume":"13","year":"1984","unstructured":"Aulakh, M. S., D. A. Rennie, and E. A. Paul. 1984. Gaseous nitrogen losses from soils under zero tillage as compared with conventional tilled system. J. Environ. 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