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C. Wong Magna Fund in Ningbo University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,7,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The emergence of multidrug-resistant\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      poses a severe threat to mariculture sustainability, highlighting the urgent need for eco-friendly antimicrobial agents. In this study, it was demonstrated that L-Cysteine (L-Cys) showed an inhibitory effect on the growth of\n                      <jats:italic toggle=\"yes\">V. parahaemolyticus<\/jats:italic>\n                      , with a minimum inhibitory concentration of 7.5 mM. Microscopic observation and viability assay revealed that L-Cys compromised bacterial membrane integrity, ultimately leading to cell death. Transcriptomic and biochemical analyses showed that L-Cys induced the reactive oxygen species (ROS) by inhibiting terminal respiratory oxidases and key antioxidant enzymes of superoxide dismutase and glutathione reductase. Also, L-Cys disrupted metabolic processes of sulfur assimilation, one-carbon metabolism, \u03b2-oxidation, and nucleotide biosynthesis, which compromised cellular repair and survival mechanisms. The inhibition of L-Cys on\n                      <jats:italic toggle=\"yes\">V. parahaemolyticus<\/jats:italic>\n                      was primarily attributed to the hydrogen sulfide generated by L-Cys metabolism, followed by an increase in ROS. In addition to its antibacterial effect, L-Cys also effectively reduced the bacterial swimming motility. Finally, L-Cys demonstrated broad-spectrum inhibitory effects on other pathogenic\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      spp. Our findings demonstrated that L-Cys is a promising antimicrobial agent that induces ROS-mediated membrane disruption, with the advantages of cost-effectiveness and environmental safety in the control of vibriosis.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Multidrug-resistant\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        poses a severe threat to mariculture sustainability and public health. Conventional antibiotics exacerbate antimicrobial resistance; therefore, in this study, we selected L-cysteine (L-Cys) as a potent inhibitor of\n                        <jats:italic toggle=\"yes\">V. parahaemolyticus<\/jats:italic>\n                        , featuring cost-effectiveness, environmental safety, and ease of large-scale production. L-Cys is metabolized into hydrogen sulfide, and the latter triggers ROS burst, and it can also disrupt metabolic processes of sulfur assimilation, one-carbon metabolism, \u03b2-oxidation, and nucleotide biosynthesis. Besides, L-Cys also significantly reduced the bacterial swimming motility. Moreover, L-Cys suppresses the growth of other pathogenic\n                        <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                        spp., offering a novel agent for vibriosis control.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00097-26","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:01:45Z","timestamp":1780491705000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["L-cysteine inhibited the growth of\n                    <i>Vibrio parahaemolyticus<\/i>\n                    via increasing the ROS level"],"prefix":"10.1128","volume":"92","author":[{"given":"Qiuyan","family":"Yang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03et85d35","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Marine Sciences, Ningbo 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