{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T01:57:47Z","timestamp":1775786267292,"version":"3.50.1"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100023082","name":"State of Washington","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023082","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2024,6,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Pododermatitis, also known as treponeme-associated hoof disease (TAHD), presents a significant challenge to elk (\n              <jats:italic>Cervus canadensis<\/jats:italic>\n              ) populations in the northwestern USA, with\n              <jats:italic>Treponema<\/jats:italic>\n              spp. consistently implicated in the lesion development. However, identifying species-specific\n              <jats:italic>Treponema<\/jats:italic>\n              strains from these lesions is hindered by its culture recalcitrance and limited genomic information. This study utilized shotgun sequencing,\n              <jats:italic>in silico<\/jats:italic>\n              genome reconstruction, and comparative genomics as a culture-independent approach to identify metagenome-assembled\n              <jats:italic>Treponema<\/jats:italic>\n              genomes (MATGs) from skin scraping samples collected from captive elk experimentally challenged with TAHD. The genomic analysis revealed 10 new MATGs, with 6 representing novel genomospecies associated with pododermatitis in elk and 4 corresponding to previously identified species\n              <jats:italic>\u2014Treponema pedis<\/jats:italic>\n              and\n              <jats:italic>Treponema phagedenis<\/jats:italic>\n              . Importantly, genomic signatures of novel genomospecies identified in this study were consistently detected in biopsy samples of free-ranging elk diagnosed with TAHD, indicating a potential etiologic association. Comparative metabolic profiling of the MATGs against other\n              <jats:italic>Treponema<\/jats:italic>\n              genomes showed a distinct metabolic profile, suggesting potential host adaptation or geographic uniqueness of these newly identified genomospecies. The discovery of novel\n              <jats:italic>Treponema<\/jats:italic>\n              genomospecies enhances our understanding of the pathogenesis of pododermatitis and lays the foundation for the development of improved molecular surveillance tools to monitor and manage the disease in free-ranging elk.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Treponema<\/jats:italic>\n                spp. play an important role in the development of pododermatitis in free-ranging elk; however, the species-specific detection of\n                <jats:italic>Treponema<\/jats:italic>\n                from pododermatitis lesions is challenging due to culture recalcitrance and limited genomic information. The study utilized shotgun sequencing and\n                <jats:italic>in silico<\/jats:italic>\n                genome reconstruction to identify novel\n                <jats:italic>Treponema<\/jats:italic>\n                genomospecies from elk with pododermatitis. The discovery of the novel\n                <jats:italic>Treponema<\/jats:italic>\n                species opens new avenues to develop molecular diagnostic and epidemiologic tools for the surveillance of pododermatitis in elk. These findings significantly enhance our understanding of the genomic landscape of the\n                <jats:italic>Treponemataceae<\/jats:italic>\n                consortium while offering valuable insights into the etiology and pathogenesis of emerging pododermatitis in elk populations.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.00105-24","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T13:00:23Z","timestamp":1715691623000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Discovery of novel treponemes associated with pododermatitis in elk (\n            <i>Cervus canadensis<\/i>\n            )"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6925-3098","authenticated-orcid":false,"given":"Sushanta","family":"Deb","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3110-3515","authenticated-orcid":true,"given":"Margaret A.","family":"Wild","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"LeClair","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-7868","authenticated-orcid":true,"given":"Devendra H.","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of 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