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Yersiniabactin was originally detected by high-pressure liquid chromatography (HPLC); commonly used PCR tests proved ineffective. Yersiniabactin production in\n            <jats:italic>P. syringae<\/jats:italic>\n            correlated with the possession of\n            <jats:italic>irp1<\/jats:italic>\n            located in a predicted yersiniabactin locus. Three similarly divergent yersiniabactin locus groups were determined: the\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            group, the\n            <jats:italic>P. syringae<\/jats:italic>\n            group, and the\n            <jats:italic>Photorhabdus luminescens<\/jats:italic>\n            group; yersiniabactin locus organization is similar in\n            <jats:italic>P. syringae<\/jats:italic>\n            and\n            <jats:italic>P. luminescens<\/jats:italic>\n            . In\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. tomato DC3000, the locus has a high GC content (63.4% compared with 58.4% for the chromosome and 60.1% and 60.7% for adjacent regions) but it lacks high-pathogenicity-island features, such as the insertion in a tRNA locus, the integrase, and insertion sequence elements. In\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. tomato DC3000 and pv. phaseolicola 1448A, the locus lies between homologues of Psyr_2284 and Psyr_2285 of\n            <jats:italic>P. syringae<\/jats:italic>\n            pv. syringae B728a, which lacks the locus. Among tested pseudomonads, a PCR test specific to two yersiniabactin locus groups detected a locus in genospecies 3, 7, and 8 of\n            <jats:italic>P. syringae<\/jats:italic>\n            , and DNA hybridization within\n            <jats:italic>P. syringae<\/jats:italic>\n            also detected a locus in the pathovars phaseolicola and glycinea. The PCR and HPLC methods enabled analysis of nonpathogenic\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . HPLC-proven yersiniabactin-producing\n            <jats:italic>E. coli<\/jats:italic>\n            lacked modifications found in\n            <jats:italic>irp1<\/jats:italic>\n            and\n            <jats:italic>irp2<\/jats:italic>\n            in the human pathogen CFT073, and it is not clear whether CFT073 produces yersiniabactin. The study provides clues about the evolution and dispersion of yersiniabactin genes. It describes methods to detect and study yersiniabactin producers, even where genes have evolved.\n          <\/jats:p>","DOI":"10.1128\/aem.00119-06","type":"journal-article","created":{"date-parts":[[2006,6,2]],"date-time":"2006-06-02T20:36:26Z","timestamp":1149280586000},"page":"3814-3825","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Yersiniabactin Production by\n            <i>Pseudomonas syringae<\/i>\n            and\n            <i>Escherichia coli<\/i>\n            , and Description of a Second Yersiniabactin Locus Evolutionary Group"],"prefix":"10.1128","volume":"72","author":[{"given":"Alain","family":"Bultreys","sequence":"first","affiliation":[{"name":"D\u00e9partement Biotechnologie, Centre Wallon de Recherches Agronomiques, B-5030 Gembloux"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Gheysen","sequence":"additional","affiliation":[{"name":"D\u00e9partement Biotechnologie, Centre Wallon de Recherches Agronomiques, B-5030 Gembloux"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmond","family":"de Hoffmann","sequence":"additional","affiliation":[{"name":"Laboratoire de Spectrom\u00e9trie de Masse, Universit\u00e9 Catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.11.5344-5351.1988"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Ausubel F. 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