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With a nested PCR assay which detects a fragment of the<jats:italic>Rickettsia<\/jats:italic>genus-specific 17-kDa antigen gene (<jats:italic>htrA<\/jats:italic>), five ticks (25%) were found to contain rickettsial DNA. One rickettsial isolate was obtained from these ticks by inoculating a suspension of a triturated tick into monolayers of Vero E6 monkey kidney cells and XTC-2 clawed toad cells, and its cell culture and genotypic characteristics were determined. Fragments of the 16S rRNA, GltA, rOmpA, rOmpB, and Sca4 genes had 100%, 100%, 99%, 99%, and 99%, respectively, nucleotide similarity to<jats:italic>Rickettsia massiliae<\/jats:italic>strain Bar29, previously isolated from<jats:italic>R. sanguineus<\/jats:italic>in Catalonia, Spain (L. Beati et al., J. Clin. Microbiol.<jats:bold>34:<\/jats:bold>2688-2694, 1996). The new isolate, AZT80, does not elicit cytotoxic effects in Vero cells and causes a persistent infection in XTC-2 cells. The AZT80 strain is susceptible to doxycycline but resistant to rifampin and erythromycin. Whether<jats:italic>R. massiliae<\/jats:italic>AZT80 is pathogenic or infectious for dogs and humans or can cause seroconversion to spotted fever group antigens in the United States is unknown.<\/jats:p>","DOI":"10.1128\/aem.00122-06","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T23:53:37Z","timestamp":1154562817000},"page":"5569-5577","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":159,"title":["Isolation and Identification of<i>Rickettsia massiliae<\/i>from<i>Rhipicephalus sanguineus<\/i>Ticks Collected in Arizona"],"prefix":"10.1128","volume":"72","author":[{"given":"Marina E.","family":"Eremeeva","sequence":"first","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Bosserman","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda J.","family":"Demma","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Zambrano","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianna M.","family":"Blau","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Dasch","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.9.5199-5201.1989"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.365"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1993.48.784"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880005202X"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.8.1922-1930.1992"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-4-839"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.11.2688-2694.1996"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"770","DOI":"10.4049\/jimmunol.90.5.770","volume":"90","year":"1963","unstructured":"Bell, E. 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