{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T01:34:05Z","timestamp":1772760845455,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Classical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFV-neutralizing antibodies and cytotoxic T lymphocyte (CTL) activities\n            <jats:italic>in vivo<\/jats:italic>\n            . Thymosin \u03b1-1 (T\u03b11), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineered\n            <jats:named-content content-type=\"genus-species\">Lactobacillus plantarum<\/jats:named-content>\n            bacteria expressing CSFV E2 protein alone (\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2) and in combination with T\u03b11 (\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2-T\u03b11) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinant\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2 and\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2-T\u03b11 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) in the levels of immune responses were observed between\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2-T\u03b11 and\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2, suggesting a better immunogenicity of\n            <jats:named-content content-type=\"genus-species\">L. plantarum<\/jats:named-content>\n            \/pYG-E2-T\u03b11 as a result of the T\u03b11 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinant\n            <jats:named-content content-type=\"genus-species\">Lactobacillus<\/jats:named-content>\n            microecological agent expressing CSFV E2 protein combined with T\u03b11 as an adjuvant provides a promising strategy for vaccine development against CSFV.\n          <\/jats:p>","DOI":"10.1128\/aem.00127-15","type":"journal-article","created":{"date-parts":[[2015,3,28]],"date-time":"2015-03-28T18:07:06Z","timestamp":1427566026000},"page":"3745-3752","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin \u03b1-1 as an Adjuvant"],"prefix":"10.1128","volume":"81","author":[{"given":"Yi-Gang","family":"Xu","sequence":"first","affiliation":[{"name":"College of Wildlife Resource, Northeast Forestry University, Harbin, China"},{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Ting","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Wildlife Resource, Northeast Forestry University, Harbin, China"},{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Technology Center, Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Yong","family":"Tian","sequence":"additional","affiliation":[{"name":"Liaoning Agricultural Technological College, Yingkou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Chun","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Wildlife Resource, Northeast Forestry University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90625-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(08)60734-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.09.043"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.2.589-597.1991"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80907-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.05.035"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2009.01.020"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.09.073"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.01.051"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.10.003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00138-3"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00137-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(88)90036-3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00497-2"},{"issue":"3","key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1099\/0022-1317-83-3-551","article-title":"Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus","volume":"83","author":"Armengol E","year":"2002","unstructured":"Armengol E, Wiesm\u00fcller K-H, Wienhold D, B\u00fcttner M, Pfaff E, Jung G, Saalm\u00fcller A. 2002. 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