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This study used a marine microalgal strain,\n              <jats:italic toggle=\"yes\">Nannochloropsis oceanica<\/jats:italic>\n              IMET1, which thrives in high CO\n              <jats:sub>2<\/jats:sub>\n              concentrations. A high-pH, high-alkalinity culture was designed for CO\n              <jats:sub>2<\/jats:sub>\n              capture through algal biomass production as well as permanent sequestration through calcium carbonate (CaCO\n              <jats:sub>3<\/jats:sub>\n              ) precipitation. This was accomplished by timed pH elevation and the addition of sodium bicarbonate to cultures of\n              <jats:italic toggle=\"yes\">N. oceanica<\/jats:italic>\n              grown at lab scale (1 L) and pilot scale (500 L) with 10% and 5% CO\n              <jats:sub>2<\/jats:sub>\n              , respectively. Our data showed that 0.02 M NaHCO\n              <jats:sub>3<\/jats:sub>\n              promoted algal growth and that sparging cultures with ambient air after 12 days raised pH and created favorable CaCO\n              <jats:sub>3<\/jats:sub>\n              formation conditions. At the 1 L scale, we reached 1.52 g L\n              <jats:sup>\u22121<\/jats:sup>\n              biomass after 12 days and an extra 9.3% CO\n              <jats:sub>2<\/jats:sub>\n              was captured in the form of CaCO\n              <jats:sub>3<\/jats:sub>\n              precipitates. At the 500 L pilot scale, an extra 60% CO\n              <jats:sub>2<\/jats:sub>\n              was captured (Day 40) with a maximum CO\n              <jats:sub>2<\/jats:sub>\n              capture rate of 63.2 g m\n              <jats:sup>\u22122<\/jats:sup>\n              day\n              <jats:sup>\u22121<\/jats:sup>\n              (Day 35). Bacterial communities associated with the microalgae were dominated by two novel Patescibacteria. Functional analysis revealed that genes for several plant growth-promotion traits (PGPTs) were enriched within this group. The microalgal-bacterial coculture system offers advantages for enhanced carbon mitigation through biomass production and simultaneous precipitation of recalcitrant CaCO\n              <jats:sub>3<\/jats:sub>\n              for long-term CO\n              <jats:sub>2<\/jats:sub>\n              storage.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Capturing carbon dioxide (CO\n                <jats:sub>2<\/jats:sub>\n                ) released from fossil fuel combustion is of the utmost importance as the impacts of climate change continue to worsen. Microalgae can remove CO\n                <jats:sub>2<\/jats:sub>\n                through their natural photosynthetic pathways and are additionally able to convert CO\n                <jats:sub>2<\/jats:sub>\n                into a stable, recalcitrant form as calcium carbonate (CaCO\n                <jats:sub>3<\/jats:sub>\n                ). We demonstrate that microalgae-based carbon capture systems can be greatly improved with high pH and high alkalinity by providing optimal conditions for carbonate precipitation. Our results with the microalga,\n                <jats:italic toggle=\"yes\">Nannochloropsis oceanica<\/jats:italic>\n                strain IMET1, show an extra 9.3% CO\n                <jats:sub>2<\/jats:sub>\n                captured as CaCO\n                <jats:sub>3<\/jats:sub>\n                at the 1 L scale and an extra 60% CO\n                <jats:sub>2<\/jats:sub>\n                captured at the 500 L (pilot) scale. Our optimized system provides a novel approach to capture CO\n                <jats:sub>2<\/jats:sub>\n                through two mechanisms: (i) as organic carbon within microalgal biomass and (ii) as inorganic carbon stored permanently in the form of CaCO\n                <jats:sub>3.<\/jats:sub>\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.00133-25","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T13:01:22Z","timestamp":1744894882000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["<i>Nannochloropsis oceanica<\/i>\n            IMET1 and its bacterial symbionts for carbon capture, utilization, and storage: biomass and calcium carbonate production under high pH and high alkalinity"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7422-2331","authenticated-orcid":false,"given":"Lauren","family":"Jonas","sequence":"first","affiliation":[{"name":"University of Maryland Center for Environmental Science","place":["Baltimore, USA"]},{"name":"Institute of Marine and Environmental Technology","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ying","family":"Lee","sequence":"additional","affiliation":[{"name":"University of Maryland Center for Environmental Science","place":["Baltimore, USA"]},{"name":"Institute of Marine and Environmental Technology","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Mroz","sequence":"additional","affiliation":[{"name":"HY-TEK Bio, LLC","place":["Baltimore, 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