{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T21:28:42Z","timestamp":1793222922032,"version":"4.5.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Genes of\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium LT2 expected to be specifically present in\n            <jats:italic>Salmonella<\/jats:italic>\n            were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of\n            <jats:italic>Salmonella<\/jats:italic>\n            serovars, including\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            subsp. I and IIIb and\n            <jats:italic>Salmonella bongori<\/jats:italic>\n            (V), and were clustered into 17 groups by their comparison patterns. A total of 38 primer pairs were constructed to represent each of the 17 groups, and PCR was performed with various\n            <jats:italic>Salmonella<\/jats:italic>\n            subspecies including\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            subsp. I, II, IIIa, IIIb, IV, VI, and V to evaluate a comprehensive DNA-based scheme for identification of\n            <jats:italic>Salmonella<\/jats:italic>\n            subspecies and the major disease-causing\n            <jats:italic>Salmonella<\/jats:italic>\n            serovars. Analysis of PCR results showed that\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            subsp. I was critically divided from other subspecies, and\n            <jats:italic>Salmonella<\/jats:italic>\n            strains belonging to\n            <jats:italic>S. enterica<\/jats:italic>\n            subsp. I were clustered based on their serovars. In addition, genotypic relationships within\n            <jats:italic>S. enterica<\/jats:italic>\n            subsp. I by PCR results were investigated. Also,\n            <jats:italic>Salmonella<\/jats:italic>\n            signature genes,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium signature genes, and\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            subsp. I signature genes were demonstrated based on their PCR results. The described PCR method suggests a rapid and convenient method for identification of\n            <jats:italic>Salmonella<\/jats:italic>\n            serovars that can be used by nonspecialized laboratories. Genome sequence comparison can be a useful tool in epidemiologic and taxonomic studies of\n            <jats:italic>Salmonella<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.00138-06","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T23:18:42Z","timestamp":1157584722000},"page":"6142-6151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Comparison of\n            <i>Salmonella enterica<\/i>\n            Serovar Typhimurium LT2 and Non-LT2\n            <i>Salmonella<\/i>\n            Genomic Sequences, and Genotyping of Salmonellae by Using PCR"],"prefix":"10.1128","volume":"72","author":[{"given":"Hyun-Joong","family":"Kim","sequence":"first","affiliation":[{"name":"Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Hong","family":"Park","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae-Yeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.4984-4991.2001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.5.1224-1230.1997"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4579-4587.1998"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5413.449"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.20.7753"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-3-601"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-6-1125"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.804-808.1996"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.2.553-563.2003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.7.1154"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.7.2330-2337.2003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02293-4"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2074-2078.2002"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.02.633-636.2002"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-38-2-129"},{"key":"e_1_3_3_18_2","first-page":"151","volume":"56","year":"2003","unstructured":"Lim, Y. 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