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In auriferous soils, the bacterium is challenged by a mixture of copper ions and gold complexes, which exert synergistic toxicity. The previously used, self-made Au(III) solution caused a synergistic toxicity of copper and gold that was based on the inhibition of the CupA-mediated efflux of cytoplasmic Cu(I) by Au(I) in this cellular compartment. In this publication, the response of the bacterium to gold and copper was investigated by using a commercially available Au(III) solution instead of the self-made solution. The new solution was five times more toxic than the previously used one. Increased toxicity was accompanied by greater accumulation of gold atoms by the cells. The contribution of copper resistance determinants to the commercially available Au(III) solution and synergistic gold-copper toxicity was studied using single- and multiple-deletion mutants. The commercially available Au(III) solution inhibited periplasmic Cu(I) homeostasis, which is required for the allocation of copper ions to copper-dependent proteins in this compartment. The presence of the gene for the periplasmic Cu(I) and Au(I) oxidase, CopA, decreased the cellular copper and gold content. Transcriptional reporter gene fusions showed that up-regulation of\n              <jats:italic>gig<\/jats:italic>\n              , encoding a minor contributor to copper resistance, was strictly glutathione dependent. Glutathione was also required to resist synergistic gold-copper toxicity. The new data indicated a second layer of synergistic copper-gold toxicity caused by the commercial Au(III) solution, inhibition of the periplasmic copper homeostasis in addition to the cytoplasmic one.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                When living in auriferous soils,\n                <jats:italic>Cupriavidus metallidurans<\/jats:italic>\n                is not only confronted with synergistic toxicity of copper ions and gold complexes but also by different gold species. A previously used gold solution made by using\n                <jats:italic>aqua regia<\/jats:italic>\n                resulted in the formation of periplasmic gold nanoparticles, and the cells were protected against gold toxicity by the periplasmic Cu(I) and Au(I) oxidase CopA. To understand the role of different gold species in the environment, another Au(III) solution was commercially acquired. This compound was more toxic due to a higher accumulation of gold atoms by the cells and inhibition of periplasmic Cu(I) homeostasis. Thus, the geo-biochemical conditions might influence Au(III) speciation. The resulting Au(III) species may subsequently interact in different ways with\n                <jats:italic>C. metallidurans<\/jats:italic>\n                and its copper homeostasis system in the cytoplasm and periplasm. This study reveals that the geochemical conditions may decide whether bacteria are able to form gold nanoparticles or not.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.00146-24","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T13:01:46Z","timestamp":1711976506000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["A gold speciation that adds a second layer to synergistic gold-copper toxicity in\n            <i>Cupriavidus metallidurans<\/i>"],"prefix":"10.1128","volume":"90","author":[{"given":"Niklas","family":"Hirth","sequence":"first","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Wiesemann","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Microscopy Unit, Biocenter, Martin Luther University Halle Wittenberg, Wittenberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle-Sophie","family":"Gerlach","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kilian","family":"Preu\u00dfner","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Galea","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Herzberg","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"},{"name":"Department of Analytical Chemistry, Helmholtz Centre for Environmental Research \u2013 UFZ, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Gro\u00dfe","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4516-8267","authenticated-orcid":true,"given":"Dietrich H.","family":"Nies","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Institute for Biology\/Microbiology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/c5mt00320b"},{"key":"e_1_3_3_3_2","first-page":"440","article-title":"Extrachromosomal inheritance controlling resistance to cadmium, cobalt and zinc ions: evidence for curing a Pseudomonas","volume":"86","author":"Mergeay M","year":"1978","unstructured":"Mergeay M, Houba C, Gerits J. 1978. 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