{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:45:24Z","timestamp":1791020724869,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A bacterium designated\n            <jats:italic>Pseudonocardia<\/jats:italic>\n            sp. strain ENV478 was isolated by enrichment culturing on tetrahydrofuran (THF) and was screened to determine its ability to degrade a range of ether pollutants. After growth on THF, strain ENV478 degraded THF (63 mg\/h\/g total suspended solids [TSS]), 1,4-dioxane (21 mg\/h\/g TSS), 1,3-dioxolane (19 mg\/h\/g TSS),\n            <jats:italic>bis<\/jats:italic>\n            -2-chloroethylether (BCEE) (12 mg\/h\/g TSS), and methyl\n            <jats:italic>tert<\/jats:italic>\n            -butyl ether (MTBE) (9.1 mg\/h\/g TSS). Although the highest rates of 1,4-dioxane degradation occurred after growth on THF, strain ENV478 also degraded 1,4-dioxane after growth on sucrose, lactate, yeast extract, 2-propanol, and propane, indicating that there was some level of constitutive degradative activity. The BCEE degradation rates were about threefold higher after growth on propane (32 mg\/h\/g TSS) than after growth on THF, and MTBE degradation resulted in accumulation of\n            <jats:italic>tert<\/jats:italic>\n            -butyl alcohol. Degradation of 1,4-dioxane resulted in accumulation of 2-hydroxyethoxyacetic acid (2HEAA). Despite its inability to grow on 1,4-dioxane, strain ENV478 degraded this compound for &gt;80 days in aquifer microcosms. Our results suggest that the inability of strain ENV478 and possibly other THF-degrading bacteria to grow on 1,4-dioxane is related to their inability to efficiently metabolize the 1,4-dioxane degradation product 2HEAA but that strain ENV478 may nonetheless be useful as a biocatalyst for remediating 1,4-dioxane-contaminated aquifers.\n          <\/jats:p>","DOI":"10.1128\/aem.00160-06","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T23:53:37Z","timestamp":1154562817000},"page":"5218-5224","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":147,"title":["Biodegradation of Ether Pollutants by\n            <i>Pseudonocardia<\/i>\n            sp. Strain ENV478"],"prefix":"10.1128","volume":"72","author":[{"given":"Simon","family":"Vainberg","sequence":"first","affiliation":[{"name":"Shaw Environmental, Inc., 17 Princess Rd., Lawrenceville, New Jersey 08648"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"McClay","sequence":"additional","affiliation":[{"name":"Shaw Environmental, Inc., 17 Princess Rd., Lawrenceville, New Jersey 08648"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisako","family":"Masuda","sequence":"additional","affiliation":[{"name":"Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, New Jersey 08901"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane","family":"Root","sequence":"additional","affiliation":[{"name":"Shaw Environmental, Inc., 304 Directors Dr., Knoxville, Tennessee 37923"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Condee","sequence":"additional","affiliation":[{"name":"Shaw Environmental, Inc., 17 Princess Rd., Lawrenceville, New Jersey 08648"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerben J.","family":"Zylstra","sequence":"additional","affiliation":[{"name":"Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, New Jersey 08901"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Steffan","sequence":"additional","affiliation":[{"name":"Shaw Environmental, Inc., 17 Princess Rd., Lawrenceville, New Jersey 08648"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00060a010"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00164711"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(77)90174-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.4.1025-1029.1993"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki038"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-003-0103-8"},{"key":"e_1_3_2_9_2","first-page":"434","year":"2002","unstructured":"DeFlaun, M. 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