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Here, we describe the identification and purification of the mutacin BHT-B-like gene locus and bacteriocin peptide from\n              <jats:italic>Streptococcus ursoris<\/jats:italic>\n              , which is closely related to\n              <jats:italic>Streptococcus ratti<\/jats:italic>\n              ; hence, we named this bacteriocin ursoricin. Ursoricin is a cationic, chromosome-encoded peptide that has potent antimicrobial effects against Gram-positive pathogens, including MRSA and VRE, with minimum inhibitory concentrations in the micromolar range. Ursoricin also inhibits the biofilm formation of high biofilm-forming\n              <jats:italic>S. aureus<\/jats:italic>\n              . Antibacterial activity was retained after treatment at 100\u00b0C for 60 min at a pH range of 3\u20139 and was partially reduced by treatment with proteinase K for 2 h (63% residual activity). The potent anti-MRSA, anti-VRE, and antibiofilm effects of ursoricin suggest that it is a possible candidate for the treatment of MRSA, VRE, and biofilm-associated infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The emergence of multidrug-resistant bacteria worldwide has posed a significant public health threat and economic burdens that make the identification and development of novel antimicrobial agents urgent. Bacteriocins are promising new agents that exhibit antibacterial activity against a wide range of human pathogens. In this study, we report that the bacteriocin produced by\n              <jats:italic>Streptococcus ursoris<\/jats:italic>\n              showed good antibacterial activity against a wide range of\n              <jats:italic>Staphylococcus aureus<\/jats:italic>\n              and enterococcus strains, particularly methicillin-resistant\n              <jats:italic>Staphylococcus aureus<\/jats:italic>\n              , vancomycin-resistant enterococci, and high biofilm-forming\n              <jats:italic>S. aureus<\/jats:italic>\n              . Interestingly, this bacteriocin had a stronger effect on\n              <jats:italic>S. aureus<\/jats:italic>\n              than on\n              <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n              , which is a major commensal bacterium in human skin; this result is important when considering the disturbance of bacterial flora, especially on the skin, mediated by the application of antibacterial agents.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.00162-24","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T13:01:10Z","timestamp":1716382870000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Ursoricin, a bacteriocin of\n            <i>Streptococcus ursoris<\/i>\n            , has potent activity against methicillin-resistant\n            <i>Staphylococcus aureus<\/i>\n            and vancomycin-resistant enterococci"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3450-1135","authenticated-orcid":false,"given":"Chutian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"},{"name":"Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9394-6107","authenticated-orcid":false,"given":"Mi Nguyen-Tra","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan"},{"name":"Project Research Centre for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, 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