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We describe a new technique using quantitative real-time PCR (qPCR) that can be used to measure in vitro and in vivo laboratory infections with\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            . We show for the first time that qPCR permits absolute quantification of the parasite while simultaneously controlling for the amount of host tissue and correlates significantly with established methods of quantification in in vitro and in vivo laboratory models of infection.\n          <\/jats:p>","DOI":"10.1128\/aem.00189-06","type":"journal-article","created":{"date-parts":[[2006,6,2]],"date-time":"2006-06-02T20:36:26Z","timestamp":1149280586000},"page":"4484-4488","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Quantification of In Vitro and In Vivo\n            <i>Cryptosporidium parvum<\/i>\n            Infection by Using Real-Time PCR"],"prefix":"10.1128","volume":"72","author":[{"given":"Nihal T.","family":"Godiwala","sequence":"first","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Boston, Massachusetts 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Vandewalle","sequence":"additional","affiliation":[{"name":"INSERM, U773, Centre de Recherche Biom\u00e9dicale Bichat-Beaujon (CRB3), BP 416, F-75018 Paris Universit\u00e9 Paris 7\u2014Denis Diderot, site Bichat, F-75870 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honorine D.","family":"Ward","sequence":"additional","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Boston, Massachusetts 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett A.","family":"Leav","sequence":"additional","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Boston, Massachusetts 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2864-2871.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.270.6.C1666"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4437-4442.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4108-4116.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.603-607.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.3.1495-1500.2005"},{"key":"e_1_3_2_8_2","first-page":"100S","volume":"38","year":"1991","unstructured":"Enriquez, F. 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