{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:04Z","timestamp":1787016544319,"version":"build-2736575974"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T00:00:00Z","timestamp":1431648000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Understanding of the colonization process of epithelial bacteria attached to the rumen tissue during rumen development is very limited. Ruminal epithelial bacterial colonization is of great significance for the relationship between the microbiota and the host and can influence the early development and health of the host. MiSeq sequencing of 16S rRNA genes and quantitative real-time PCR (qPCR) were applied to characterize ruminal epithelial bacterial diversity during rumen development in this study. Seventeen goat kids were selected to reflect the no-rumination (0 and 7 days), transition (28 and 42 days), and rumination (70 days) phases of animal development. Alpha diversity indices (operational taxonomic unit [OTU] numbers, Chao estimate, and Shannon index) increased (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01) with age, and principal coordinate analysis (PCoA) revealed that the samples clustered together according to age group. Phylogenetic analysis revealed that\n                    <jats:named-content content-type=\"genus-species\">Proteobacteria<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Firmicutes<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Bacteroidetes<\/jats:named-content>\n                    were detected as the dominant phyla regardless of the age group, and the abundance of\n                    <jats:named-content content-type=\"genus-species\">Proteobacteria<\/jats:named-content>\n                    declined quadratically with age (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), while the abundances of\n                    <jats:named-content content-type=\"genus-species\">Bacteroidetes<\/jats:named-content>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.088) and\n                    <jats:named-content content-type=\"genus-species\">Firmicutes<\/jats:named-content>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.009) increased with age. At the genus level,\n                    <jats:named-content content-type=\"genus-species\">Escherichia<\/jats:named-content>\n                    (80.79%) dominated at day zero, while\n                    <jats:named-content content-type=\"genus-species\">Prevotella<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Butyrivibrio<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Campylobacter<\/jats:named-content>\n                    surged (linearly;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01) in abundance at 42 and 70 days. qPCR showed that the total copy number of epithelial bacteria increased linearly (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.013) with age. In addition, the abundances of the genera\n                    <jats:named-content content-type=\"genus-species\">Butyrivibrio<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Campylobacter<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Desulfobulbus<\/jats:named-content>\n                    were positively correlated with rumen weight, rumen papilla length, ruminal ammonia and total volatile fatty acid concentrations, and activities of carboxymethylcellulase (CMCase) and xylanase. Taking the data together, colonization by ruminal epithelial bacteria is age related (achieved at 2 months) and might participate in the anatomic and functional development of the rumen.\n                  <\/jats:p>","DOI":"10.1128\/aem.00203-15","type":"journal-article","created":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T22:25:41Z","timestamp":1426285541000},"page":"3502-3509","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Taxonomic Identification of Ruminal Epithelial Bacterial Diversity during Rumen Development in Goats"],"prefix":"10.1128","volume":"81","author":[{"given":"Jinzhen","family":"Jiao","sequence":"first","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, People's Republic of China"},{"name":"Graduate University of Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, People's Republic of China"},{"name":"Graduate University of Chinese Academy of Sciences, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanshe","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/32.1.139"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.04.032"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013607"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731109990681"},{"key":"e_1_3_3_6_2","first-page":"92","article-title":"16S rDNA analysis of bacterial diversity in three fractions of cow rumen","volume":"16","author":"Cho SJ","year":"2006","unstructured":"Cho SJ, Cho KM, Shin EC, Lim WJ, Hong SY, Choi BR, Kang JM, Lee SM, Kim YH, Kim H, Yun HD. 2006. 16S rDNA analysis of bacterial diversity in three fractions of cow rumen. 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