{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:47:26Z","timestamp":1790232446483,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extended-spectrum \u03b2-lactamase (ESBL)-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            strains are believed to be widely distributed among humans and animals; however, to date, there are only few studies that support this assumption on a regional or countrywide scale. Therefore, a study was designed to assess the prevalence of ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            in dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs, and dust samples were analyzed for ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            using selective media. PCR was performed to screen for CTX-M and\n            <jats:italic>ampC<\/jats:italic>\n            resistance genes. A total of 598 samples yielded 196 (32.8%) that contained ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            , originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            positive than samples from beef cattle farms (fecal samples,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001; boot swabs,\n            <jats:italic>P<\/jats:italic>\n            = 0.014; and dust samples,\n            <jats:italic>P<\/jats:italic>\n            = 0.041). A total of 183 isolates (93.4%) of 196 ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            -positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty-six additional isolates contained\n            <jats:italic>ampC<\/jats:italic>\n            genes, and 5 of the 46 isolates expressed a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            gene. The study shows that ESBL-producing\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            strains are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            in cattle in Germany.\n          <\/jats:p>","DOI":"10.1128\/aem.00204-13","type":"journal-article","created":{"date-parts":[[2013,3,2]],"date-time":"2013-03-02T12:00:28Z","timestamp":1362225628000},"page":"3027-3032","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":127,"title":["Prevalence of Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms"],"prefix":"10.1128","volume":"79","author":[{"given":"A.","family":"Schmid","sequence":"first","affiliation":[{"name":"Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"},{"name":"Clinic for Ruminants, LMU Munich, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"H\u00f6rmansdorfer","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Messelh\u00e4usser","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"K\u00e4sbohrer","sequence":"additional","affiliation":[{"name":"The Federal Institute for Risk Assessment, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Sauter-Louis","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, LMU Munich, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Mansfeld","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, LMU Munich, Oberschleissheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh395"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00247-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.833-835.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh444"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00559-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.08.016"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.6.2056-2058.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02831-10"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2006.12.145"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0615"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03001.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl048"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01851.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2716-2722.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.8.3732-3734.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(07)70854-3"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46909-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00404-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.005850-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.783"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.1570"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-21"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02299-07"},{"key":"e_1_3_2_25_2","unstructured":"DANMAP.\n2012.\nDANMAP 2011\u2014Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals food and humans in Denmark.\nhttp:\/\/www.danmap.org\/Downloads\/\u223c\/media\/Projekt%20sites\/Danmap\/DANMAP%20reports\/Danmap_2011.ashx.\nLast accessed January 2013."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02239-05"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.10.1696"},{"key":"e_1_3_2_28_2","first-page":"1","article-title":"Scientific opinion on the public health risks of bacterial strains producing extended-spectrum \u03b2-lactamases and\/or AmpC \u03b2-lactamases in food and food-producing animals","volume":"9","author":"EFSA","year":"2011","unstructured":"EFSA. 2011. 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