{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:22:47Z","timestamp":1791418967065,"version":"4.3.3"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study integrated metagenomic and nuclear magnetic resonance (NMR) spectroscopic approaches to investigate microbial metabolic potential for organic matter decomposition and nitrogen (N) and phosphorus (P) acquisition in soils of an ombrotrophic peatland in the Marcell Experimental Forest (MEF), Minnesota, USA. This analysis revealed vertical stratification in key enzymatic pathways and taxa containing these pathways. Metagenomic analyses revealed that genes encoding laccases and dioxygenases, involved in aromatic compound degradation, declined in relative abundance with depth, while the relative abundance of genes encoding metabolism of amino sugars and all four saccharide groups increased with depth in parallel with a 50% reduction in carbohydrate content. Most Cu-oxidases were closely related to genes from\n                    <jats:named-content content-type=\"genus-species\">Proteobacteria<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Acidobacteria<\/jats:named-content>\n                    , and type 4 laccase-like Cu-oxidase genes were &gt;8 times more abundant than type 3 genes, suggesting an important and overlooked role for type 4 Cu-oxidase in phenolic compound degradation. Genes associated with sulfate reduction and methanogenesis were the most abundant anaerobic respiration genes in these systems, with low levels of detection observed for genes of denitrification and Fe(III) reduction. Fermentation genes increased in relative abundance with depth and were largely affiliated with\n                    <jats:named-content content-type=\"genus-species\">Syntrophobacter<\/jats:named-content>\n                    .\n                    <jats:named-content content-type=\"genus-species\">Methylocystaceae<\/jats:named-content>\n                    -like small-subunit (SSU) rRNA genes,\n                    <jats:italic>pmoA<\/jats:italic>\n                    , and\n                    <jats:italic>mmoX<\/jats:italic>\n                    genes were more abundant among methanotrophs. Genes encoding N\n                    <jats:sub>2<\/jats:sub>\n                    fixation, P uptake, and P regulons were significantly enriched in the surface peat and in comparison to other ecosystems, indicating N and P limitation. Persistence of inorganic orthophosphate throughout the peat profile in this P-limiting environment indicates that P may be bound to recalcitrant organic compounds, thus limiting P bioavailability in the subsurface. Comparative metagenomic analysis revealed a high metabolic potential for P transport and starvation, N\n                    <jats:sub>2<\/jats:sub>\n                    fixation, and oligosaccharide degradation at MEF relative to other wetland and soil environments, consistent with the nutrient-poor and carbohydrate-rich conditions found in this\n                    <jats:named-content content-type=\"genus-species\">Sphagnum<\/jats:named-content>\n                    -dominated boreal peatland.\n                  <\/jats:p>","DOI":"10.1128\/aem.00206-14","type":"journal-article","created":{"date-parts":[[2014,3,29]],"date-time":"2014-03-29T02:11:29Z","timestamp":1396059089000},"page":"3531-3540","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Microbial Metabolic Potential for Carbon Degradation and Nutrient (Nitrogen and Phosphorus) Acquisition in an Ombrotrophic Peatland"],"prefix":"10.1128","volume":"80","author":[{"given":"Xueju","family":"Lin","sequence":"first","affiliation":[{"name":"Georgia Institute of Technology, Schools of Biology and Earth &amp; Atmospheric Sciences, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malak M.","family":"Tfaily","sequence":"additional","affiliation":[{"name":"Florida State University, Department of Earth, Ocean and Atmospheric Science, Tallahassee, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan J.","family":"Green","sequence":"additional","affiliation":[{"name":"DNA Services Facility, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Megan","family":"Steinweg","sequence":"additional","affiliation":[{"name":"Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Chanton","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology, Schools of Biology and Earth &amp; Atmospheric Sciences, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aopeau","family":"Imvittaya","sequence":"additional","affiliation":[{"name":"Florida State University, Department of Chemistry &amp; Biochemistry, Tallahassee, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey P.","family":"Chanton","sequence":"additional","affiliation":[{"name":"Florida State University, Department of Earth, Ocean and Atmospheric Science, Tallahassee, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Cooper","sequence":"additional","affiliation":[{"name":"Florida State University, Department of Chemistry &amp; Biochemistry, Tallahassee, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Schadt","sequence":"additional","affiliation":[{"name":"Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel E.","family":"Kostka","sequence":"additional","affiliation":[{"name":"Georgia Institute of Technology, Schools of Biology and Earth &amp; Atmospheric Sciences, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1672\/0277-5212(2006)26[889:TCBONA]2.0.CO;2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.5194\/bg-5-1475-2008"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35051650"},{"key":"e_1_3_3_5_2","first-page":"125","volume-title":"Ecological studies","author":"Moore TR","year":"2006","unstructured":"MooreTRBasilikoN. 2006. Decomposition in boreal peatlands, p 125\u2013143. In WiederRKVittDH (ed), Ecological studies, vol 188. Boreal peatland ecosystems. Springer-Verlag, Berlin, Germany."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1174268"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8817.2003.00783.x"},{"key":"e_1_3_3_8_2","first-page":"309","article-title":"Towards \u201cTera-Terra\u201d: terabase sequencing of terrestrial metagenomes","volume":"6","author":"Jansson J","year":"2011","unstructured":"JanssonJ. 2011. Towards \u201cTera-Terra\u201d: terabase sequencing of terrestrial metagenomes. Microbe 6:309\u2013315.","journal-title":"Microbe"},{"key":"e_1_3_3_9_2","first-page":"64","article-title":"Modeling the Earth microbiome","volume":"7","author":"Gilbert J","year":"2012","unstructured":"GilbertJMeyerF. 2012. Modeling the Earth microbiome. 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