{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:29:20Z","timestamp":1785421760698,"version":"3.56.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Zhiyuan Science Foundation of BIPT","award":["2023004"],"award-info":[{"award-number":["2023004"]}]},{"name":"Intergovernmental International Cooperation on Science and Technology Innovation of National Key Research and Development Project of China","award":["2023YFE0122000"],"award-info":[{"award-number":["2023YFE0122000"]}]},{"name":"Open Funding of State Key Laboratory of Coal Mine Disaster Prevention and Control","award":["2024SKLKF03"],"award-info":[{"award-number":["2024SKLKF03"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42177220"],"award-info":[{"award-number":["42177220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42421003, 22536002"],"award-info":[{"award-number":["42421003, 22536002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Frontiers Science Center of Polar Science","award":["SOO2025-01"],"award-info":[{"award-number":["SOO2025-01"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,6,17]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Dehalococcoides<\/jats:italic>\n (\n Dhc<\/jats:italic>\n ) has recently gained significant attention in microbial reductive dehalogenation due to its specialized ability to metabolize various halocarbons. While previous reports have documented\n Dhc<\/jats:italic>\n -mediated dihaloelimination of 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,2-dibromoethane, this study reveals an expansion of\n Dhc<\/jats:italic>\n \u2019s dihaloelimination substrate range. We successfully established PJ\n DCA<\/jats:sub>\n and PJ\n TCA<\/jats:sub>\n enrichment cultures from petroleum-contaminated soil, exhibiting dihaloelimination activity toward 1,1,2-trichloroethane (1,1,2-TCA), 1,2,3-trichloropropane (1,2,3-TCP), and 1,1,2,2-tetrachloroethane (1,1,2,2-TeCA)\u2014substrates previously only known to be transformed by other organohalide-respiring bacteria (OHRB). Amplicon sequencing revealed the predominance of\n Dhc<\/jats:italic>\n as the primary OHRB within the PJ\n DCA<\/jats:sub>\n and PJ\n TCA<\/jats:sub>\n cultures, leading to the identification of two novel\n Dhc<\/jats:italic>\n populations, designated as strains PJ\n DCA<\/jats:sub>\n and PJ\n TCA<\/jats:sub>\n , respectively. These strains showed robust growth yields of 4.1 \u00b1 0.4 \u00d7 10\n 7<\/jats:sup>\n and 7.6 \u00b1 0.4 \u00d7 10\n 7<\/jats:sup>\n cells per \u03bcmol Cl\n \u2212<\/jats:sup>\n released when using 1,2-DCA and 1,1,2-TCA as electron acceptors, respectively. Genomic analysis revealed a reductive dehalogenase (RDase) homologous to the characterized DcpA, an enzyme known for dihaloelimination activity. Proteomic studies confirmed the expression of this DcpA-like RDase during dihaloelimination of 1,2-DCA, 1,1,2-TCA, and 1,2-DCP. This work identifies\n Dhc<\/jats:italic>\n strains that retain dihaloelimination activity toward classical substrates while expanding this capability to 1,1,2-TCA, 1,2,3-TCP, and 1,1,2,2-TeCA. The discovery of these versatile\n Dhc<\/jats:italic>\n strains enhances our understanding of microbial dehalogenation potential across diverse geological settings and improves prospects for bioremediation of halogenated alkane contaminants.\n <\/jats:p>\n <\/jats:sec>\n \n IMPORTANCE<\/jats:title>\n \n This study identifies\n Dehalococcoides<\/jats:italic>\n strains capable of dihaloeliminating diverse chlorinated alkanes (1,1,2-TCA, 1,2,3-TCP, 1,1,2,2-TeCA), expanding their bioremediation potential. These compounds are persistent groundwater contaminants with high toxicity. The discovery of\n Dhc<\/jats:italic>\n populations (PJ\n DCA<\/jats:sub>\n \/PJ\n TCA<\/jats:sub>\n ) with robust growth yields (10\n 7<\/jats:sup>\n cells\/\u03bcmol Cl\n \u2212<\/jats:sup>\n ) and broad substrate range offers new solutions for detoxifying complex halogenated pollutant mixtures. The identification and proteomic confirmation of DcpA-like RDases (DheA) provide the genetic and functional basis for this expanded dihaloelimination capacity. These findings advance strategies for\n in situ<\/jats:italic>\n remediation of industrial sites contaminated with C\n 2<\/jats:sub>\n -C\n 3<\/jats:sub>\n halocarbons, reducing ecological and human health risks.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/aem.00209-26","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T13:00:16Z","timestamp":1778158816000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Revisiting the dihaloelimination potential of\n Dehalococcoides<\/i>\n revealed by genomic and proteomic analyses"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3391-9980","authenticated-orcid":true,"given":"Xiaocui","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences","place":["Shenyang, China"]},{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongming","family":"Cai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/012tb2g32","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Earth System Science, Tianjin University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences","place":["Shenyang, China"]},{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-0355","authenticated-orcid":true,"given":"Jiubin","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/012tb2g32","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Earth System Science, Tianjin University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8151-3697","authenticated-orcid":true,"given":"Tong","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Environmental Science and Engineering, Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwei","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/025s55q11","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Environmental Engineering, Beijing Institute of Petrochemical Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongming","family":"Xiu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qbk4x57","id-type":"ROR","asserted-by":"publisher"}],"name":"Zhejiang Key Laboratory of Environment and Health of New Pollutants, School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences","place":["Hangzhou, 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