{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T21:54:14Z","timestamp":1776722054005,"version":"3.51.2"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2010,8,15]],"date-time":"2010-08-15T00:00:00Z","timestamp":1281830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The model rhizobacterium\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            KT2440 and other fluorescent pseudomonads possess two bacterioferritins, Bfr\u03b1 and Bfr\u03b2. However, the regulatory systems controlling the expression of these genes and the roles of these proteins in iron homeostasis are ill defined. Our studies show that both\n            <jats:italic>bfr<\/jats:italic>\n            \u03b1 and\n            <jats:italic>bfr<\/jats:italic>\n            \u03b2 were monocistronic: promoter motifs and transcriptional start sites were identified, and Fur boxes and \u03c3\n            <jats:sup>S<\/jats:sup>\n            -dependent regulatory motifs were absent. The expressions of\n            <jats:italic>bfr<\/jats:italic>\n            \u03b1 and\n            <jats:italic>bfr<\/jats:italic>\n            \u03b2 were enhanced by iron exposure and were maximal in cells rapidly growing in a high-iron environment. Both\n            <jats:italic>bfr<\/jats:italic>\n            \u03b1 and\n            <jats:italic>bfr<\/jats:italic>\n            \u03b2 were positively regulated by Fur, and both were expressed independently of adjoining, functionally related genes. The loss of Bfr\u03b1 or Bfr\u03b2 individually resulted in a significant reduction (ca. 17%) in cellular iron levels, and the deletion of both\n            <jats:italic>bfr<\/jats:italic>\n            \u03b1 and\n            <jats:italic>bfr<\/jats:italic>\n            \u03b2 reduced cellular iron levels by 38% relative to those of the wild type. The mutants varied in their abilities to grow in low-iron medium; while growths (rate and final cell density) of single mutants and the wild type were similar, that of the double mutant was reduced significantly. Mutants lacking Bfr\u03b1 and\/or Bfr\u03b2 showed no change relative to the wild type in sensitivity to reactive oxygen species toxicity. Collectively, the data show that while Bfr\u03b1 and Bfr\u03b2 could function independently of each other, an interaction-dependent function cannot be ruled out. Furthermore, regardless of the mechanism, a primary benefit of the bacterioferritins to\n            <jats:italic>P. putida<\/jats:italic>\n            KT2440 appears to be the enhancement of its survival in the environment by strengthening its tolerance to iron starvation.\n          <\/jats:p>","DOI":"10.1128\/aem.00215-10","type":"journal-article","created":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T00:23:19Z","timestamp":1277079799000},"page":"5335-5343","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Molecular Analysis of Two Bacterioferritin Genes,\n            <i>bfr<\/i>\n            \u03b1 and\n            <i>bfr<\/i>\n            \u03b2, in the Model Rhizobacterium\n            <i>Pseudomonas putida<\/i>\n            KT2440"],"prefix":"10.1128","volume":"76","author":[{"given":"Shicheng","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Soil Science, University of Wisconsin\u2014Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William F.","family":"Bleam","sequence":"additional","affiliation":[{"name":"Department of Soil Science, University of Wisconsin\u2014Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Hickey","sequence":"additional","affiliation":[{"name":"Department of Soil Science, University of Wisconsin\u2014Madison, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.5.1415-1428.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(08)60134-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi035253u"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504266102"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00424-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.2.279"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01640-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg215"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4118"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-145-10-2967"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01823-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-008-9193-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807749200"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10038-X"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01910-07"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9600862"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2003.00465.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.12.3842-3850.1990"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00130-9"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.042770"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7186-7195.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3730-3742.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.03.013"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032066599"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00095-0"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0038-0717(99)00156-X"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3040493"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00366.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.4409"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707840200"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3570241"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0602-z"},{"key":"e_1_3_2_35_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.85-93.1996"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1183-784"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408410490435151"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01803-08"},{"key":"e_1_3_2_40_2","first-page":"35","year":"1998","unstructured":"Temple, L. M., A. E. Sage, H. P. Schweizer, and P. V. Phibbs, Jr. 1998. Carbohydrate catabolism in Pseudomonas aeruginosa, p. 35-72. In T. C. Montie (ed.), Pseudomonas. 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