{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:02:17Z","timestamp":1790326937327,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Listeriosis is a disease that causes significant economic losses at the farm level because of high morbidity and mortality in ruminants. This study was performed to investigate the role of ruminants in the epidemiology of listeriosis in northern Italy and the possible association of animal-adapted strains of<jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>with strains associated with human disease. Twenty ruminant rhombencephalitis isolates previously confirmed as<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>by bacteriology and PCR were characterized by serotyping, pulsed-field gel electrophoresis, multi-virulence-locus sequence typing (MVLST), and multiplex single nucleotide polymorphism (mSNP) typing for the detection of epidemic clones. Subtyping results were subsequently compared with those obtained from human, food, and environmental isolates of<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>, including 311 isolates from the University of Turin, Grugliasco, Italy, and 165 isolates representing major human listeriosis outbreaks worldwide, in addition to other unrelated isolates. Both mSNP typing and MVLST showed that 60% of the isolates analyzed belonged to epidemic clone I (ECI), which has been epidemiologically linked to several human outbreaks of listeriosis. In particular, the 1981 Canada outbreak was linked to the use of sheep manure and the 1985 California outbreak was linked to the use of raw cow's milk. In our study, ECI isolates were collected from different ruminant species on geographically and temporally distinct occasions for the last 13 years. Our results support the hypothesis that ruminants represent possible natural reservoirs of<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>strains capable of causing epidemics of listeriosis in humans.<\/jats:p>","DOI":"10.1128\/aem.00219-13","type":"journal-article","created":{"date-parts":[[2013,3,2]],"date-time":"2013-03-02T12:00:28Z","timestamp":1362225628000},"page":"3059-3066","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Ruminant Rhombencephalitis-Associated Listeria monocytogenes Strains Constitute a Genetically Homogeneous Group Related to Human Outbreak Strains"],"prefix":"10.1128","volume":"79","author":[{"given":"Paulo Ricardo Dell'Armelina","family":"Rocha","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Lomonaco","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Bottero","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Dalmasso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Dondo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Grattarola","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Zuccon","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Iulini","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen John","family":"Knabel","sequence":"additional","affiliation":[{"name":"Department of Food Science, Penn State University, University Park, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Capucchio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Casalone","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-0233(97)80005-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.05.002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2012.03.009"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.11.1811"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.4.616"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.991-996.1994"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000146"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420051414.ch11"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032896"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.10.022"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.757-762.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.2.913-920.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420051414.ch7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0988-x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02553-10"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2009.03.003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042448"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00102-09"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-005-0054-9"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01575-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06185-11"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1901.121167"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.10.5893-5899.2005"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300130"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.06.018"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2005.00866.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870201400407"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.2460\/javma.1999.215.03.369","article-title":"Molecular investigation of a listeriosis outbreak in goats caused by an unusual strain of Listeria monocytogenes","volume":"215","author":"Wiedmann M","year":"1999","unstructured":"WiedmannM MobiniS ColeJRJr WatsonCK JeffersGT BoorKJ . 1999. Molecular investigation of a listeriosis outbreak in goats caused by an unusual strain of Listeria monocytogenes. J. Am. Vet. Med. Assoc. 215: 369\u2013 371.","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06507-11"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.6.1624-1629.1991"},{"key":"e_1_3_2_33_2","first-page":"195","article-title":"Characterization of Listeria monocytogenes isolates from cattle and ground beef by pulsed-field gel electrophoresis","volume":"44","author":"Foerster C","year":"2012","unstructured":"FoersterC VidalL TroncosoM FigueroaG . 2012. Characterization of Listeria monocytogenes isolates from cattle and ground beef by pulsed-field gel electrophoresis. Rev. Argent. Microbiol. 44: 195\u2013 200.","journal-title":"Rev. Argent. Microbiol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/632513"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.04.016"},{"key":"e_1_3_2_36_2","first-page":"L147\/1","article-title":"Regulation (EC) No 999\/2001\/EC of the European Parliament and of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies","volume":"44","author":"European Parliament","year":"2001","unstructured":"European Parliament. 2001. Regulation (EC) No 999\/2001\/EC of the European Parliament and of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. Official J. Eur. Communities 44: L147\/1\u2013 L147\/40. http:\/\/eur-lex.europa.eu\/LexUriServ\/LexUriServ.do?uri=OJ:L:2001:147:0001:0040:EN:PDF.","journal-title":"Official J. Eur. Communities"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2009.00292.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3819-3822.2004"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0456"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00429-11"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00501-8"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-0031.2010.00329.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_3_2_44_2","volume-title":"R: a language and environment for statistical computing, reference index version 2.2.1","author":"R Development Core Team","year":"2005","unstructured":"R Development Core Team. 2005. R: a language and environment for statistical computing, reference index version 2.2.1. R Foundation for Statistical Computing, Vienna, Austria."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2005.tb00519.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.116.9.233"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-008-0049-0"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.3.574"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-5-1095"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.9"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2350-2355.2005"},{"key":"e_1_3_2_53_2","first-page":"1357","article-title":"Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe\u2014United States, August-September 2011","volume":"60","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention. 2011. Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe\u2014United States, August-September 2011. MMWR Morb. Mortal. Wkly. Rep. 60: 1357\u2013 1358.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1086\/428575"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.7.1680"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.5833-5841.2004"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3818"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00961-07"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.8.4458-4467.2004"}],"updated-by":[{"DOI":"10.1128\/aem.02923-13","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000}}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00219-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00219-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T20:08:43Z","timestamp":1715026123000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00219-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":58,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1128\/AEM.00219-13"],"URL":"https:\/\/doi.org\/10.1128\/aem.00219-13","relation":{"correction":[{"id-type":"doi","id":"10.1128\/AEM.02923-13","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5]]},"assertion":[{"value":"2013-01-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}