{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T22:47:49Z","timestamp":1785538069821,"version":"3.56.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T00:00:00Z","timestamp":1400112000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            During fungal rock phosphate (RP) solubilization, a significant quantity of fluoride (F\n            <jats:sup>\u2212<\/jats:sup>\n            ) is released together with phosphorus (P), strongly inhibiting the process. In the present study, the effect of two F\n            <jats:sup>\u2212<\/jats:sup>\n            adsorbents [activated alumina (Al\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>3<\/jats:sub>\n            ) and biochar] on RP solubilization by\n            <jats:named-content content-type=\"genus-species\">Aspergillus niger<\/jats:named-content>\n            was examined. Al\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>3<\/jats:sub>\n            adsorbed part of the F\n            <jats:sup>\u2212<\/jats:sup>\n            released but also adsorbed soluble P, which makes it inappropriate for microbial RP solubilization systems. In contrast, biochar adsorbed only F\n            <jats:sup>\u2212<\/jats:sup>\n            while enhancing phosphate solubilization 3-fold, leading to the accumulation of up to 160 mg of P per liter. By comparing the values of F\n            <jats:sup>\u2212<\/jats:sup>\n            measured in solution at the end of incubation and those from a predictive model, it was estimated that up to 19 mg of F\n            <jats:sup>\u2212<\/jats:sup>\n            per liter can be removed from solution by biochar when added at 3 g liter\n            <jats:sup>\u22121<\/jats:sup>\n            to the culture medium. Thus, biochar acted as an F\n            <jats:sup>\u2212<\/jats:sup>\n            sink during RP solubilization and led to an F\n            <jats:sup>\u2212<\/jats:sup>\n            concentration in solution that was less inhibitory to the process. In the presence of biochar,\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            produced larger amounts of citric, gluconic, and oxalic acids, whether RP was present or not. Our results show that biochar enhances RP solubilization through two interrelated processes: partial removal of the released F\n            <jats:sup>\u2212<\/jats:sup>\n            and increased organic acid production. Given the importance of organic acids for P solubilization and that most of the RPs contain high concentrations of F\n            <jats:sup>\u2212<\/jats:sup>\n            , the proposed solubilization system offers an important technological improvement for the microbial production of soluble P fertilizers from RP.\n          <\/jats:p>","DOI":"10.1128\/aem.00241-14","type":"journal-article","created":{"date-parts":[[2014,3,8]],"date-time":"2014-03-08T05:40:12Z","timestamp":1394257212000},"page":"3081-3085","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Biochar Enhances Aspergillus niger Rock Phosphate Solubilization by Increasing Organic Acid Production and Alleviating Fluoride Toxicity"],"prefix":"10.1128","volume":"80","author":[{"given":"Gilberto de Oliveira","family":"Mendes","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Lopez","family":"Zafra","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica, Facultad de Ciencias, Universidad de Granada, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolay Bojkov","family":"Vassilev","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica, Facultad de Ciencias, Universidad de Granada, Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo Ribeiro","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Solos, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, Brazil"},{"name":"Pesquisador Bolsista do Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Bras\u00edlia, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Jos\u00e9 Ivo","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Departamento de Estat\u00edstica, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maur\u00edcio Dutra","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, Brazil"},{"name":"Pesquisador Bolsista do Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Bras\u00edlia, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/su3102027"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2011.01.052"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6130"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11104-006-9054-y"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-012-1156-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1193-1250"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01487-13"},{"key":"e_1_3_2_9_2","first-page":"397","volume-title":"Ullmann's encyclopedia of industrial chemistry","author":"Aigueperse J","year":"2000","unstructured":"AigueperseJMollardPDevilliersDChemlaMFaronRRomanoRCuerJP. 2000. 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