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Initial tests showed that\n              <jats:italic>Bifidobacterium longum<\/jats:italic>\n              subsp.\n              <jats:italic>longum<\/jats:italic>\n              strains boosted the growth of DSM 17938 during\n              <jats:italic>in vivo-<\/jats:italic>\n              like conditions. Further characterization revealed that one of the strains, BG-L47, had better bile and acid tolerance compared to BG-L48, as well as mucus adhesion compared to both BG-L48 and the control strain BB536. BG-L47 also had the capacity to metabolize a broad range of carbohydrates and sugar alcohols. Mapping of glycoside hydrolase (GH) genes of BG-L47 and BB536 revealed many GHs associated with plant-fiber utilization. However, BG-L47 had a broader phenotypic fiber utilization capacity. In addition,\n              <jats:italic>B. longum<\/jats:italic>\n              subsp.\n              <jats:italic>longum<\/jats:italic>\n              cells boosted the bioactivity of extracellular membrane vesicles (MV) produced by\n              <jats:italic>L. reuteri<\/jats:italic>\n              DSM 17938 during co-cultivation. Secreted 5\u2032-nucleotidase (5\u2032NT), an enzyme that converts AMP into the signal molecule adenosine, was increased in MV boosted by BG-L47. The MV exerted an improved antagonistic effect on the pain receptor transient receptor potential vanilloid 1 (TRPV1) and increased the expression of the immune development markers IL-6 and IL-1\u00df in a peripheral blood mononuclear cell (PBMC) model. Finally, the safety of BG-L47 was evaluated both by genome safety assessment and in a human safety study. Microbiota analysis showed that the treatment did not induce significant changes in the composition. In conclusion,\n              <jats:italic>B. longum<\/jats:italic>\n              subsp.\n              <jats:italic>longum<\/jats:italic>\n              BG-L47 has favorable physiological properties, can boost the\n              <jats:italic>in vitro<\/jats:italic>\n              activity of\n              <jats:italic>L. reuteri<\/jats:italic>\n              DSM 17938, and is safe for consumption, making it a candidate for further evaluation in probiotic studies.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              By using probiotics that contain a combination of strains with synergistic properties, the likelihood of achieving beneficial interactions with the host can increase. In this study, we first performed a broad screening of\n              <jats:italic>Bifidobacterium longum<\/jats:italic>\n              subsp.\n              <jats:italic>longum<\/jats:italic>\n              strains in terms of synergistic potential and physiological properties. We identified a superior strain, BG-L47, with favorable characteristics and potential to boost the activity of the known probiotic strain\n              <jats:italic>Limosilactobacillus reuteri<\/jats:italic>\n              DSM 17938. Furthermore, we demonstrated that BG-L47 is safe for consumption in a human randomized clinical study and by performing a genome safety assessment. This work illustrates that bacteria\u2013bacteria interactions differ at the strain level and further provides a strategy for finding and selecting companion strains of probiotics.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.00247-24","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T13:03:29Z","timestamp":1718715809000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["<i>Bifidobacterium longum<\/i>\n            subsp.\n            <i>longum<\/i>\n            BG-L47 boosts growth and activity of\n            <i>Limosilactobacillus reuteri<\/i>\n            DSM 17938 and its extracellular membrane vesicles"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5983-1771","authenticated-orcid":true,"given":"Ludwig","family":"Ermann Lundberg","sequence":"first","affiliation":[{"name":"Department of Molecular Sciences, 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