{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:33:53Z","timestamp":1774924433693,"version":"3.50.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Blastomyces dermatitidis<\/jats:named-content>\n            , a thermally dimorphic fungus, is the etiologic agent of North American blastomycosis. Clinical presentation is varied, ranging from silent infections to fulminant respiratory disease and dissemination to skin and other sites. Exploration of the population genetic structure of\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            would improve our knowledge regarding variation in virulence phenotypes, geographic distribution, and difference in host specificity. The objective of this study was to develop and test a panel of microsatellite markers to delineate the population genetic structure within a group of clinical and environmental isolates of\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            . We developed 27 microsatellite markers and genotyped\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            isolates from various hosts and environmental sources (\n            <jats:italic>n<\/jats:italic>\n            =112). Assembly of a neighbor-joining tree of allele-sharing distance revealed two genetically distinct groups, separated by a deep node. Bayesian admixture analysis showed that two populations were statistically supported. Principal coordinate analysis also reinforced support for two genetic groups, with the primary axis explaining 61.41% of the genetic variability. Group 1 isolates average 1.8 alleles\/locus, whereas group 2 isolates are highly polymorphic, averaging 8.2 alleles\/locus. In this data set, alleles at three loci are unshared between the two groups and appear diagnostic. The mating type of individual isolates was determined by PCR. Both mating type-specific genes, the HMG and \u03b1-box domains, were represented in each of the genetic groups, with slightly more isolates having the HMG allele. One interpretation of this study is that the species currently designated\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            includes a cryptic subspecies or perhaps a separate species.\n          <\/jats:p>","DOI":"10.1128\/aem.00258-11","type":"journal-article","created":{"date-parts":[[2011,6,25]],"date-time":"2011-06-25T23:16:27Z","timestamp":1309043787000},"page":"5123-5131","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Population Genetic Structure of Clinical and Environmental Isolates of Blastomyces dermatitidis, Based on 27 Polymorphic Microsatellite Markers"],"prefix":"10.1128","volume":"77","author":[{"given":"Jennifer K.","family":"Meece","sequence":"first","affiliation":[{"name":"Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew C.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Imperial College Faculty of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Henk","sequence":"additional","affiliation":[{"name":"Imperial College Faculty of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian L.","family":"Sloss","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, Wisconsin Cooperative Fishery Research Unit, College of Natural Resources, University of Wisconsin Stevens Point, Stevens Point, Wisconsin 54481"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt D.","family":"Reed","sequence":"additional","affiliation":[{"name":"Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219780001521"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/j.1365-280X.1999.00214.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.12.004"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/368455a0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(02)00038-7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00020-07"},{"key":"e_1_3_3_8_2","first-page":"841","article-title":"Fixed-bin analysis for statistical evaluation of continuous distributions of allelic data from VNTR loci, for use in forensic comparisons","volume":"48","author":"Budowle B.","year":"1991","unstructured":"BudowleB.. 1991. 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