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Here, we designed and tested an alternative\n              <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n              diagnostic test, a fluorescent probe-based, loop-mediated isothermal amplification (P-LAMP) assay that also targets IS\n              <jats:italic toggle=\"yes\">2404<\/jats:italic>\n              . Benchmarked against IS\n              <jats:italic toggle=\"yes\">2404<\/jats:italic>\n              qPCR, P-LAMP was equally specific and nearly as sensitive (analytical sensitivity of four vs two\n              <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n              genome copies). Clinical and environmental specimen validation against IS\n              <jats:italic toggle=\"yes\">2404<\/jats:italic>\n              qPCR showed P-LAMP had 100% sensitivity and specificity. P-LAMP was twice as fast as qPCR with an average time-to-positive at the limit-of-detection of 19 minutes. P-LAMP targeting IS\n              <jats:italic toggle=\"yes\">2404<\/jats:italic>\n              is a versatile assay that addresses the performance issues of previously described IS\n              <jats:italic toggle=\"yes\">2404<\/jats:italic>\n              LAMP formats. This study tackles a key research priority for Buruli ulcer and represents another avenue for the development of rapid and accessible molecular diagnostics for this neglected tropical disease.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Buruli ulcer is a neglected tropical disease caused by infection with\n                <jats:italic toggle=\"yes\">Mycobacterium ulcerans<\/jats:italic>\n                . Correct diagnosis is essential before appropriate treatment for Buruli ulcer can be started. Development of a portable, easy-to-use diagnostic test for\n                <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                has been identified by the World Health Organization as a research priority. Buruli ulcer most commonly occurs in remote, rural areas; therefore, an ideal test is one that can be used at (or near) the point of care (community health centres) without the need for specialized laboratories. Here, we describe a molecular test using loop-mediated isothermal amplification (LAMP) to detect DNA specific to\n                <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                and show that this new test has equivalent performance to the gold standard\n                <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                PCR test currently used worldwide. Our new test is rapid (30 minutes to run), simple to perform, and could be further developed into a robust, portable format to provide accessible and affordable\n                <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                diagnostics anywhere.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/aem.00270-25","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T13:00:33Z","timestamp":1744808433000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Detection of\n            <i>Mycobacterium ulcerans<\/i>\n            with IS\n            <i>2404<\/i>\n            loop-mediated isothermal amplification and a fluorescent reporter probe"],"prefix":"10.1128","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-8503","authenticated-orcid":true,"given":"Jean Y. 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