{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:11:48Z","timestamp":1790107908341,"version":"4.0.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Eighty-one tetracycline-resistant\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    sp. strains were isolated from farm-raised catfish. Morphological and biochemical characteristics indicated that 23 of the 81 aeromonads were\n                    <jats:italic>Aeromonas hydrophila<\/jats:italic>\n                    , 7 isolates were\n                    <jats:italic>Aeromonas trota<\/jats:italic>\n                    , 6 isolates were\n                    <jats:italic>Aeromonas caviae<\/jats:italic>\n                    , 42 isolates were\n                    <jats:italic>Aeromonas veronii<\/jats:italic>\n                    , and 3 isolates were\n                    <jats:italic>Aeromonas jandaei<\/jats:italic>\n                    . However, the AluI and MboI restriction fragment length polymorphism (RFLP) patterns of the PCR-amplified 1.4-kb 16S rRNA gene from all 81 tetracycline-resistant aeromonads from catfish were identical to the RFLP banding patterns of\n                    <jats:italic>A. veronii<\/jats:italic>\n                    ATCC 35626, indicating that all 81 isolates were strains of\n                    <jats:italic>A. veronii<\/jats:italic>\n                    . A multiplex PCR assay successfully amplified the 5 tetracycline-resistant genes (\n                    <jats:italic>tet<\/jats:italic>\n                    A to E) from the genomic DNA of all 81 isolates. The assay determined that\n                    <jats:italic>tet<\/jats:italic>\n                    E was the dominant gene occurring in 73\/81 (90.0%) of the aeromonads. Plasmids (2.0 to 20 kb) were isolated from 33 of the 81 isolates. Dendrogram analysis of the SpeI pulsed-field gel electrophoresis identified 15 distinct macrorestriction patterns among the isolates. Our results indicate the need for use of 16S rRNA in the identification of\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    spp. and the prevalence of catfish as a reservoir of\n                    <jats:italic>tet<\/jats:italic>\n                    genes.\n                  <\/jats:p>","DOI":"10.1128\/aem.00271-06","type":"journal-article","created":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T13:50:39Z","timestamp":1159969839000},"page":"6461-6466","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Biochemical and Molecular Characterization of Tetracycline-Resistant\n                    <i>Aeromonas veronii<\/i>\n                    Isolates from Catfish"],"prefix":"10.1128","volume":"72","author":[{"given":"Mohamed","family":"Nawaz","sequence":"first","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kidon","family":"Sung","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Steele","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.1103-1104.1998"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4194-4201.1998"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.3.908-912.1994"},{"key":"e_1_3_1_5_2","unstructured":"Aquaculture Outlook. 2001. 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