{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:09:44Z","timestamp":1761293384090},"reference-count":46,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Recently, our group along with others reported that the\n Vibrio<\/jats:named-content>\n FadR regulatory protein is unusual in that, unlike the prototypical\n fadR<\/jats:italic>\n product of\n Escherichia coli<\/jats:named-content>\n , which has only one ligand-binding site,\n Vibrio<\/jats:named-content>\n FadR has two ligand-binding sites and represents a new mechanism for fatty acid sensing. The promoter region of the\n vc2105<\/jats:italic>\n gene, encoding a putative thioesterase, was mapped, and a putative FadR-binding site (AA CTG GTA AGA GCA CTT) was proposed. Different versions of the FadR regulatory proteins were prepared and purified to homogeneity. Both electrophoretic mobility shift assay (EMSA) and surface plasmon resonance (SPR) determined the direct interaction of the\n vc2105<\/jats:italic>\n gene with FadR proteins of various origins. Further, EMSAs illustrated that the addition of long-chain acyl-coenzyme A (CoA) species efficiently dissociates the\n vc2105<\/jats:italic>\n promoter from the FadR regulator. The expression level of the\n Vibrio cholerae vc2105<\/jats:named-content>\n gene was elevated 2- to 3-fold in a\n fadR<\/jats:italic>\n null mutant strain, validating that FadR is a repressor for the\n vc2105<\/jats:italic>\n gene. The \u03b2-galactosidase activity of a\n vc2105-lacZ<\/jats:italic>\n transcriptional fusion was increased over 2-fold upon supplementation of growth medium with oleic acid. Unlike the\n fadD<\/jats:italic>\n gene, a member of the\n Vibrio fad<\/jats:italic>\n regulon, the VC2105 protein played no role in bacterial growth and virulence-associated gene expression of\n ctxAB<\/jats:italic>\n (cholera toxin A\/B) and\n tcpA<\/jats:italic>\n (toxin coregulated pilus A). Given that the transcriptional regulation of\n vc2105<\/jats:italic>\n fits the criteria for fatty acid degradation (\n fad<\/jats:italic>\n ) genes, we suggested that it is a new member of the\n Vibrio fad<\/jats:italic>\n regulon.\n <\/jats:p>\n \n IMPORTANCE<\/jats:bold>\n The\n Vibrio<\/jats:named-content>\n FadR regulator is unusual in that it has two ligand-binding sites. Different versions of the FadR regulatory proteins were prepared and characterized\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n . An auxiliary\n fad<\/jats:italic>\n gene (\n vc2105<\/jats:italic>\n ) from\n Vibrio<\/jats:named-content>\n was proposed that encodes a putative thioesterase and has a predicted FadR-binding site (AAC TGG TA A GAG CAC TT). The function of this putative binding site was proved using both EMSA and SPR. Further\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n experiments revealed that the\n Vibrio<\/jats:named-content>\n FadR is a repressor for the\n vc2105<\/jats:italic>\n gene. Unlike\n fadD<\/jats:italic>\n , a member of the\n Vibrio fad<\/jats:italic>\n regulon, VC2105 played no role in bacterial growth and expression of the two virulence-associated genes (\n ctxAB<\/jats:italic>\n and\n tcpA<\/jats:italic>\n ). Therefore, since transcriptional regulation of\n vc2105<\/jats:italic>\n fits the criteria for\n fad<\/jats:italic>\n genes, it seems likely that\n vc2105<\/jats:italic>\n acts as a new auxiliary member of the\n Vibrio fad<\/jats:italic>\n regulon.\n <\/jats:p>","DOI":"10.1128\/aem.00293-16","type":"journal-article","created":{"date-parts":[[2016,3,5]],"date-time":"2016-03-05T03:19:34Z","timestamp":1457147974000},"page":"2819-2832","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Transcriptional Repression of the VC2105 Protein by Vibrio FadR Suggests that It Is a New Auxiliary Member of the\n fad<\/i>\n Regulon"],"prefix":"10.1128","volume":"82","author":[{"given":"Rongsui","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingxia","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youjun","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 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