{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:01:50Z","timestamp":1791140510947,"version":"4.1.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2010,10,15]],"date-time":"2010-10-15T00:00:00Z","timestamp":1287100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Microcin J25 (MccJ25) is a plasmid-encoded, 21-amino-acid, antibacterial peptide produced by<jats:italic>Escherichia coli<\/jats:italic>. MccJ25 inhibits RNA polymerase and the membrane respiratory chain. MccJ25 uptake into<jats:italic>E. coli<\/jats:italic>-sensitive strains is mediated by the outer membrane receptor FhuA and the inner membrane proteins TonB, ExbB, ExbD, and SbmA. This peptide is active on some<jats:italic>E. coli<\/jats:italic>,<jats:italic>Salmonella<\/jats:italic>, and<jats:italic>Shigella<\/jats:italic>species strains, while other Gram-negative bacteria, such as clinical isolates of<jats:italic>Enterobacter cloacae<\/jats:italic>,<jats:italic>Citrobacter freundii<\/jats:italic>,<jats:italic>Klebsiella pneumoniae<\/jats:italic>,<jats:italic>Pseudomonas aeruginosa<\/jats:italic>,<jats:italic>Acinetobacter baumannii<\/jats:italic>,<jats:italic>Moraxella catarrhalis<\/jats:italic>, and<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium, are completely resistant. In the present work, we demonstrated that the membrane-permeabilizing peptide (KFF)<jats:sub>3<\/jats:sub>K made some resistant strains sensitive to MccJ25, among them<jats:italic>S.<\/jats:italic>Typhimurium, where the antibiotic inhibits<jats:italic>in vitro<\/jats:italic>cell growth and bacterial replication within macrophages. The results demonstrate that the membrane permeabilization induced by (KFF)<jats:sub>3<\/jats:sub>K allows MccJ25 penetration in an FhuA and SbmA-independent manner and suggest that the combination of both peptides could be considered as a therapeutic agent against pathogenic<jats:italic>Salmonella<\/jats:italic>strains.<\/jats:p>","DOI":"10.1128\/aem.00307-10","type":"journal-article","created":{"date-parts":[[2010,8,28]],"date-time":"2010-08-28T02:30:37Z","timestamp":1282962637000},"page":"6837-6842","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Sensitization of Microcin J25-Resistant Strains by a Membrane-Permeabilizing Peptide"],"prefix":"10.1128","volume":"76","author":[{"given":"Mar\u00eda Fernanda","family":"Pomares","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica de la Nutrici\u00f3n, Instituto Superior de Investigaciones Biol\u00f3gicas (Concejo Nacional de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica-Universidad Nacional de Tucum\u00e1n) and Instituto de Qu\u00edmica Biologica Dr. Bernabe Bloj, Chacabuco 461, 4000 San Miguel de Tucum\u00e1n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica A.","family":"Delgado","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica de la Nutrici\u00f3n, Instituto Superior de Investigaciones Biol\u00f3gicas (Concejo Nacional de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica-Universidad Nacional de Tucum\u00e1n) and Instituto de Qu\u00edmica Biologica Dr. Bernabe Bloj, Chacabuco 461, 4000 San Miguel de Tucum\u00e1n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia S.","family":"Corbal\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica de la Nutrici\u00f3n, Instituto Superior de Investigaciones Biol\u00f3gicas (Concejo Nacional de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica-Universidad Nacional de Tucum\u00e1n) and Instituto de Qu\u00edmica Biologica Dr. Bernabe Bloj, Chacabuco 461, 4000 San Miguel de Tucum\u00e1n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo N.","family":"Far\u00edas","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica de la Nutrici\u00f3n, Instituto Superior de Investigaciones Biol\u00f3gicas (Concejo Nacional de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica-Universidad Nacional de Tucum\u00e1n) and Instituto de Qu\u00edmica Biologica Dr. Bernabe Bloj, Chacabuco 461, 4000 San Miguel de Tucum\u00e1n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula A.","family":"Vincent","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica de la Nutrici\u00f3n, Instituto Superior de Investigaciones Biol\u00f3gicas (Concejo Nacional de Investigaci\u00f3n Cient\u00edfica y T\u00e9cnica-Universidad Nacional de Tucum\u00e1n) and Instituto de Qu\u00edmica Biologica Dr. Bernabe Bloj, Chacabuco 461, 4000 San Miguel de Tucum\u00e1n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja036677e"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00206-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.10.186"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1046\/j.1432-1327.1999.00085.x","volume":"259","year":"1999","unstructured":"Blond, A., J. 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