{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:16:15Z","timestamp":1791663375335,"version":"4.3.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Center for Advanced Processing and Packaging Studies","award":["GRT865889"],"award-info":[{"award-number":["GRT865889"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            , vancomycin-resistant strains of\n            <jats:named-content content-type=\"genus-species\">Enterococcus faecalis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Lactobacillus plantarum<\/jats:named-content>\n            , and other Gram-positive bacteria. The producer strain, designated OSY-I\n            <jats:sub>1<\/jats:sub>\n            , was determined to be\n            <jats:named-content content-type=\"genus-species\">Brevibacillus laterosporus<\/jats:named-content>\n            via morphological, biochemical, and genetic analyses. The antimicrobial agent was extracted from cells of OSY-I\n            <jats:sub>1<\/jats:sub>\n            with isopropanol, purified by high-performance liquid chromatography, and structurally analyzed using mass spectrometry (MS) and nuclear magnetic resonance (NMR). The MS and NMR results, taken together, uncovered a linear lipopeptide consisting of 13 amino acids and an N-terminal C\n            <jats:sub>6<\/jats:sub>\n            fatty acid (FA) chain, 2-hydroxy-3-methylpentanoic acid. The lipopeptide (FA-Dhb-Leu-Orn-Ile-Ile-Val-Lys-Val-Val-Lys-Tyr-Leu-valinol, where Dhb is \u03b1,\u03b2-didehydrobutyric acid and valinol is 2-amino-3-methyl-1-butanol) has a molecular mass of 1,583.0794 Da and contains three modified amino acid residues: \u03b1,\u03b2-didehydrobutyric acid, ornithine, and valinol. The compound, designated brevibacillin, was determined to be a member of a cationic lipopeptide antibiotic family. In addition to its potency against drug-resistant bacteria, brevibacillin also exhibited low MICs (1 to 8 \u03bcg\/ml) against selected foodborne pathogenic and spoilage bacteria, such as\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Bacillus cereus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Alicyclobacillus acidoterrestris<\/jats:named-content>\n            . Purified brevibacillin showed no sign of degradation when it was held at 80\u00b0C for 60 min, and it retained at least 50% of its antimicrobial activity when it was held for 22 h under acidic or alkaline conditions. On the basis of these findings, brevibacillin is a potent antimicrobial lipopeptide which is potentially useful to combat drug-resistant bacterial pathogens and foodborne pathogenic and spoilage bacteria.\n          <\/jats:p>","DOI":"10.1128\/aem.00315-16","type":"journal-article","created":{"date-parts":[[2016,2,27]],"date-time":"2016-02-27T03:39:35Z","timestamp":1456544375000},"page":"2763-2772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria"],"prefix":"10.1128","volume":"82","author":[{"given":"Xu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, The Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6386-2156","authenticated-orcid":false,"given":"En","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, The Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Yuan","sequence":"additional","affiliation":[{"name":"Nuclear Magnetic Resonance Facility, Campus Chemical Instrument Center, The Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Proteomics Facility, Campus Chemical Instrument Center, The Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed E.","family":"Yousef","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, The Ohio State University, Columbus, Ohio, USA"},{"name":"Department of Microbiology, The Ohio State University, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.02.007"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.02.006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.06.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2005.04.002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70143-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07782-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02023-06"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-013-0099-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/insects4030476"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6657-6664.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2010.03.002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.01.001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/np070209r"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.18426"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14472-8"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh546"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-006-9041-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606129103"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3623-3630.2000"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1051\/epn\/19861701011"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.1698"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00404272"},{"key":"e_1_3_2_25_2","volume-title":"Performance standards for antimicrobial disc susceptibility tests; approved standard. CLSI document M02-A10","author":"Clinical and Laboratory Standards Institute","year":"2009","unstructured":"Clinical and Laboratory Standards Institute. 2009. Performance standards for antimicrobial disc susceptibility tests; approved standard. CLSI document M02-A10. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-4337.2003.tb00017.x"},{"key":"e_1_3_2_27_2","unstructured":"O'Donnell BJ. October 1995. Method of inhibiting fungi by Bacillus laterosporus. US patent 5 455 028A."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-008-9730-6"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00092-5"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol006942q"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo060667p"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8519-8530.2005"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03775-13"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(01)00498-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612023395475"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.03.010"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00315-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00315-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T18:24:43Z","timestamp":1645640683000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00315-16"}},"subtitle":[],"editor":[{"given":"C. M.","family":"Dozois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1128\/AEM.00315-16"],"URL":"https:\/\/doi.org\/10.1128\/aem.00315-16","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"2016-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}