{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:54:28Z","timestamp":1791424468861,"version":"4.3.3"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Region Grand Est"},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-15-CE21-0007"],"award-info":[{"award-number":["ANR-15-CE21-0007"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006488","name":"Institut National de la Recherche Agronomique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Integrative and conjugative elements (ICEs) are widespread chromosomal mobile genetic elements which can transfer autonomously by conjugation in bacteria. Thirteen ICEs with a conjugation module closely related to that of ICE\n            <jats:italic>St3<\/jats:italic>\n            of\n            <jats:named-content content-type=\"genus-species\">Streptococcus thermophilus<\/jats:named-content>\n            were characterized in\n            <jats:named-content content-type=\"genus-species\">Streptococcus salivarius<\/jats:named-content>\n            by whole-genome sequencing. Sequence comparison highlighted ICE evolution by shuffling of 3 different integration\/excision modules (for integration in the 3\u2032 end of the\n            <jats:italic>fda<\/jats:italic>\n            ,\n            <jats:italic>rpsI<\/jats:italic>\n            , or\n            <jats:italic>rpmG<\/jats:italic>\n            gene) with the conjugation module of the ICE\n            <jats:italic>St3<\/jats:italic>\n            subfamily. Sequence analyses also pointed out a recombination occurring at\n            <jats:italic>oriT<\/jats:italic>\n            (likely mediated by the relaxase) as a mechanism of ICE evolution. Despite a similar organization in two operons including three conserved genes, the regulation modules show a high diversity (about 50% amino acid sequence divergence for the encoded regulators and presence of unrelated additional genes) with a probable impact on the regulation of ICE activity. Concerning the accessory genes, ICEs of the ICE\n            <jats:italic>St3<\/jats:italic>\n            subfamily appear particularly rich in restriction-modification systems and orphan methyltransferase genes. Other cargo genes that could confer a selective advantage to the cell hosting the ICE were identified, in particular, genes for bacteriocin synthesis and cadmium resistance. The functionality of 2 ICEs of\n            <jats:named-content content-type=\"genus-species\">S. salivarius<\/jats:named-content>\n            was investigated. Autonomous conjugative transfer to other\n            <jats:named-content content-type=\"genus-species\">S. salivarius<\/jats:named-content>\n            strains, to\n            <jats:named-content content-type=\"genus-species\">S. thermophilus<\/jats:named-content>\n            , and to\n            <jats:named-content content-type=\"genus-species\">Enterococcus faecalis<\/jats:named-content>\n            was observed. The analysis of the ICE-\n            <jats:italic>fda<\/jats:italic>\n            border sequence in these transconjugants allowed the localization of the DNA cutting site of the ICE integrase.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The ICE\n            <jats:italic>St3<\/jats:italic>\n            subfamily of ICEs appears to be widespread in streptococci and targets diverse chromosomal integration sites. These ICEs carry diverse cargo genes that can confer a selective advantage to the host strain. The maintenance of these mobile genetic elements likely relies in part on self-encoded restriction-modification systems. In this study, intra- and interspecies transfer was demonstrated for 2 ICEs of\n            <jats:named-content content-type=\"genus-species\">S. salivarius<\/jats:named-content>\n            . Closely related ICEs were also detected\n            <jats:italic>in silico<\/jats:italic>\n            in other\n            <jats:named-content content-type=\"genus-species\">Streptococcus<\/jats:named-content>\n            species (\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">S. parasanguinis<\/jats:named-content>\n            ), thus indicating that diffusion of ICE\n            <jats:italic>St3<\/jats:italic>\n            -related elements probably plays a significant role in horizontal gene transfer (HGT) occurring in the oral cavity but also in the digestive tract, where\n            <jats:named-content content-type=\"genus-species\">S. salivarius<\/jats:named-content>\n            is present.\n          <\/jats:p>","DOI":"10.1128\/aem.00337-17","type":"journal-article","created":{"date-parts":[[2017,4,22]],"date-time":"2017-04-22T00:35:15Z","timestamp":1492821315000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Diversity of Integrative and Conjugative Elements of Streptococcus salivarius and Their Intra- and Interspecies Transfer"],"prefix":"10.1128","volume":"83","author":[{"given":"Narimane","family":"Dahmane","sequence":"first","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Libante","sequence":"additional","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Charron-Bourgoin","sequence":"additional","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Gu\u00e9don","sequence":"additional","affiliation":[{"name":"STLO, Agrocampus Ouest, INRA, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Gu\u00e9don","sequence":"additional","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Leblond-Bourget","sequence":"additional","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7266-3071","authenticated-orcid":false,"given":"Sophie","family":"Payot","sequence":"additional","affiliation":[{"name":"DynAMic, Universit\u00e9 de Lorraine, INRA, Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116871109"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35012500"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03014.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12058"},{"key":"e_1_3_3_6_2","volume-title":"Bacterial integrative mobile genetic elements","author":"Roberts AP","year":"2013","unstructured":"Roberts AP MullanyP. 2013. 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