{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:37:25Z","timestamp":1792679845136,"version":"4.5.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A\n            <jats:italic>mer-lux<\/jats:italic>\n            bioreporter was constructed to assess the bioavailability of methylmercury [CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II)] in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            . The bioreporter was shown to be sensitive, with a detection limit of 2.5 nM CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II), and was used to investigate the effects of chlorides, humic acids, and thiols on the bioavailability of CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II) in\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            . It was found that increasing the concentration of chlorides resulted in an increase in CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II) bioavailability, suggesting that there was passive diffusion of the neutral complex (CH\n            <jats:sub>3<\/jats:sub>\n            HgCl\n            <jats:sup>0<\/jats:sup>\n            ). Humic acids were found to reduce the bioavailability of CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II) in varying degrees. Complexation with cysteine resulted in increased bioavailability of CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II), while assays with equivalent concentrations of methionine and leucine had little or no effect on bioavailability. The mechanism of uptake of the mercurial-cysteine complexes is likely not passive diffusion but could result from the activities of a cysteine transport system. The bioavailability of CH\n            <jats:sub>3<\/jats:sub>\n            Hg(II) decreased with increasing glutathione concentrations.\n          <\/jats:p>","DOI":"10.1128\/aem.00362-12","type":"journal-article","created":{"date-parts":[[2012,8,4]],"date-time":"2012-08-04T04:24:53Z","timestamp":1344054293000},"page":"7276-7282","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Effect of Inorganic and Organic Ligands on the Bioavailability of Methylmercury as Determined by Using a\n            <i>mer-lux<\/i>\n            Bioreporter"],"prefix":"10.1128","volume":"78","author":[{"given":"Udonna","family":"Ndu","sequence":"first","affiliation":[{"name":"Department of Marine Sciences, University of Connecticut, Groton, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Marine Sciences, University of Connecticut, Groton, Connecticut, USA"},{"name":"Department of Chemistry, University of Connecticut, Groton, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Marine Sciences, University of Connecticut, Groton, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senjie","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Marine Sciences, University of Connecticut, Groton, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter T.","family":"Visscher","sequence":"additional","affiliation":[{"name":"Department of Marine Sciences, University of Connecticut, Groton, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/es011044q"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00046-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.11.4267-4271.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44579-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac199567071117"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr.2006.09.003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2670(86)80026-5"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/abio.200390018"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr050353m"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.32.1.114-120.1976"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2307\/4589366"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marchem.2005.07.004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2009.09.030"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/es960318k"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/es034302n"},{"key":"e_1_3_2_17_2","first-page":"247","article-title":"Methylmercury accumulation and fluxes across the intestine of channel catfish, Ictalurus punctatus","volume":"132","author":"Leaner JJ","year":"2002","unstructured":"LeanerJJ MasonRP. 2002. 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