{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:34:52Z","timestamp":1791264892332,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    beta-lactamases confer resistance to critically important cephalosporin drugs. Recovered from both hospital- and community-acquired infections,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    was first reported in U.S. livestock in 2010. It has been hypothesized that veterinary use of cephalosporins in livestock populations may lead to the dissemination of beta-lactamase-encoding genes. Therefore, our objectives were to estimate the frequency and distribution of coliform bacteria harboring\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    in the fecal flora of Ohio dairy cattle populations. In addition, we characterized the CTX-M alleles carried by the isolates, their plasmidic contexts, and the genetic diversity of the bacterial isolates themselves. We also evaluated the association between ceftiofur use and the likelihood of recovering cephalosporinase-producing bacteria. Thirty fresh fecal samples and owner-reported ceftiofur use data were collected from each of 25 Ohio dairy farms. Fecal samples (\n                    <jats:italic>n<\/jats:italic>\n                    = 747) yielded 70\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    -positive\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    isolates from 5\/25 herds, 715\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-2<\/jats:sub>\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    isolates from 25\/25 herds, and 274\n                    <jats:italic>Salmonella<\/jats:italic>\n                    spp. from 20\/25 herds. The within-herd prevalence among\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    -positive herds ranged from 3.3 to 100% of samples. Multiple pulsed-field gel electrophoresis (PFGE) patterns, plasmid replicon types, and CTX-M genes were detected. Plasmids with CTX-M-1, -15, and -14 alleles were clonal by restriction fragment length polymorphism (RFLP) within herds, and specific plasmid incompatibility group markers were consistently associated with each\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    allele. PFGE of total bacterial DNA showed similar within-herd clustering, with the exception of one herd, which revealed at least 6 different PFGE signatures. We were unable to detect an association between owner-reported ceftiofur use and the probability of recovering\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    or\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-2<\/jats:sub>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/aem.00373-12","type":"journal-article","created":{"date-parts":[[2012,4,27]],"date-time":"2012-04-27T23:53:12Z","timestamp":1335570792000},"page":"4552-4560","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle"],"prefix":"10.1128","volume":"78","author":[{"given":"Dixie F.","family":"Mollenkopf","sequence":"first","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew F.","family":"Weeman","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua B.","family":"Daniels","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie J.","family":"Abley","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Mathews","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wondwossen A.","family":"Gebreyes","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wittum","sequence":"additional","affiliation":[{"name":"Departments of Veterinary Preventive Medicine, The Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1139\/w01-139"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00055-09"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01707-08"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1207.051555"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00354-08"},{"key":"e_1_3_1_8_2","unstructured":"Centers for Disease Control and Prevention (ed). 1996 (updated 2000). 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