{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:28:40Z","timestamp":1791232120229,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A striking feature of\n            <jats:named-content content-type=\"genus-species\">Burkholderia contaminans<\/jats:named-content>\n            strain MS14 is the production of a glycolipopeptide named occidiofungin. Occidiofungin has a broad range of antifungal activities against plant and animal pathogens. In this study, a complete covalent structure characterization and identification of the whole genomic DNA region for the occidiofungin gene (\n            <jats:italic>ocf<\/jats:italic>\n            ) cluster are described. Discovery of the presence of 2,4-diaminobutyric acid and 3-chloro-\u03b2-hydroxytyrosine and elucidation of the structure of a novel C\n            <jats:sub>18<\/jats:sub>\n            fatty amino acid residue have been achieved. In addition, seven additional putative open reading frames (the genes from\n            <jats:italic>ocfI<\/jats:italic>\n            to\n            <jats:italic>ocfN<\/jats:italic>\n            [\n            <jats:italic>ocfI-N<\/jats:italic>\n            ] and ORF16) were identified. Transcription of all the putative genes\n            <jats:italic>ocfI-N<\/jats:italic>\n            identified in the region except ORF16 was regulated by both\n            <jats:italic>ambR1<\/jats:italic>\n            and\n            <jats:italic>ambR2<\/jats:italic>\n            . Elucidation of the structure and the\n            <jats:italic>ocf<\/jats:italic>\n            gene cluster provides insight into the biosynthesis of occidiofungin and promotes future aims at understanding the biosynthetic machinery. This work provides new avenues for optimizing the production and synthesis of structural analogs of occidiofungin.\n          <\/jats:p>","DOI":"10.1128\/aem.00377-11","type":"journal-article","created":{"date-parts":[[2011,7,9]],"date-time":"2011-07-09T03:15:08Z","timestamp":1310181308000},"page":"6189-6198","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Genetic and Biochemical Map for the Biosynthesis of Occidiofungin, an Antifungal Produced by Burkholderia contaminans Strain MS14"],"prefix":"10.1128","volume":"77","author":[{"given":"Ganyu","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 32 Creelman St., Mississippi State, Mississippi 39762"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Texas A&amp;M University, College Station, Texas 77843"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aixin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 32 Creelman St., Mississippi State, Mississippi 39762"},{"name":"Department of Plant Pathology, Shandong Agricultural University, Taian, China 271018"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-En","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 32 Creelman St., Mississippi State, Mississippi 39762"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"52","article-title":"Three kanamycin resistance gene cassettes with different polylinkers","volume":"18","author":"Alexeyev M. 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