{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:16:20Z","timestamp":1791101780650,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Tick-borne encephalitis (TBE), a viral infection of the central nervous system, is endemic in many Eurasian countries. In Switzerland, TBE risk areas have been characterized by geographic mapping of clinical cases. Since mass vaccination should significantly decrease the number of TBE cases, alternative methods for exposure risk assessment are required. We established a new PCR-based test for the detection of TBE virus (TBEV) in ticks. The protocol involves an automated, high-throughput nucleic acid extraction method (QIAsymphony SP system) and a one-step duplex real-time reverse transcription-PCR (RT-PCR) assay for the detection of European subtype TBEV, including an internal process control. High usability, reproducibility, and equivalent performance for virus concentrations down to 5 \u00d7 10\n            <jats:sup>3<\/jats:sup>\n            viral genome equivalents\/\u03bcl favor the automated protocol compared to the modified guanidinium thiocyanate-phenol-chloroform extraction procedure. The real-time RT-PCR allows fast, sensitive (limit of detection, 10 RNA copies\/\u03bcl), and specific (no false-positive test results for other TBEV subtypes, other flaviviruses, or other tick-transmitted pathogens) detection of European subtype TBEV. The new detection method was applied in a national surveillance study, in which 62,343\n            <jats:italic>Ixodes ricinus<\/jats:italic>\n            ticks were screened for the presence of TBE virus. A total of 38 foci of endemicity could be identified, with a mean virus prevalence of 0.46%. The foci do not fully agree with those defined by disease mapping. Therefore, the proposed molecular test procedure constitutes a prerequisite for an appropriate TBE surveillance. Our data are a unique complement of human TBE disease case mapping in Switzerland.\n          <\/jats:p>","DOI":"10.1128\/aem.00391-10","type":"journal-article","created":{"date-parts":[[2010,5,8]],"date-time":"2010-05-08T02:25:30Z","timestamp":1273285530000},"page":"4241-4249","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["High-Throughput Procedure for Tick Surveys of Tick-Borne Encephalitis Virus and Its Application in a National Surveillance Study in Switzerland"],"prefix":"10.1128","volume":"76","author":[{"given":"Rahel","family":"Ga\u0308umann","sequence":"first","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Mu\u0308hlemann","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Strasser","sequence":"additional","affiliation":[{"name":"Spiez Laboratory, Federal Office for Civil Protection, Spiez, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian M.","family":"Beuret","sequence":"additional","affiliation":[{"name":"Spiez Laboratory, Federal Office for Civil Protection, Spiez, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(99)80009-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81783-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.01022.x"},{"key":"e_1_3_2_5_2","first-page":"156","volume":"162","year":"1987","unstructured":"Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem.162:156-159.","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/47.1.89"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2002.060"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.09.001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","unstructured":"Euro Surveill. 2008 13","DOI":"10.2807\/ese.13.43.19012-en"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.4.1822-1826.1989"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/515195"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-1-179"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/sim.4780111206"},{"key":"e_1_3_2_14_2","unstructured":"Infectious diseases\u2014information on tick-borne encephalitis. 2009"},{"key":"e_1_3_2_15_2","first-page":"115","volume":"41","year":"1997","unstructured":"Gresikova, M., and M. Kaluzova. 1997. Biology of tick-borne encephalitis virus. Acta Virol.41:115-124.","journal-title":"Acta Virol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000149880"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.25-36.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(02)00206-1"},{"key":"e_1_3_2_19_2","first-page":"859","year":"2000","unstructured":"Heinz, F. X., M. S. Collett, R. H. Purcell, E. A. Gould, C. R. Howard, M. Houghton, R. J. M. Moormann, C. M. Rice, and H. J. Thiel. 2000. Family Flaviviridae, p. 859-878. In M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. Carstens, M. K. Estes, S. Lemon, J. Maniloff, M. A. Mayo, D. McGeogch, C. R. Pringle, and R. B. Wickner (ed.), Virus taxonomy. Seventh report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, CA.","journal-title":"Virus taxonomy."},{"key":"e_1_3_2_20_2","unstructured":"Epidemiology of TBE. 2009"},{"key":"e_1_3_2_21_2","first-page":"307","volume":"42","year":"1995","unstructured":"Korenberg, E. I., and Y. V. Kovalevskii. 1995. Variation in parameters affecting risk of human disease due to TBE virus. Folia Parasitol. (Praha)42:307-312.","journal-title":"Folia Parasitol. (Praha)"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8622"},{"key":"e_1_3_2_23_2","unstructured":"Amplification methods. 2008"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.3.2027-2031.1996"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/42.6.1063"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(02)80005-4"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000072780.66347.B1"},{"key":"e_1_3_2_28_2","first-page":"3","volume":"9","year":"1954","unstructured":"Pospisil, L., L. Jandasek, and J. Pesek. 1954. Isolation of new strains of meningoencephalitis virus in the Brno region during the summer of 1953. Lek List.9:3-5. (In Czech.)","journal-title":"Lek List."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0928-0197(95)00022-4"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)01423-4"},{"key":"e_1_3_2_31_2","first-page":"1179","volume":"88","year":"1949","unstructured":"Rampas, J., and F. Gallia. 1949. Isolation of tick-borne encephalitis virus from ticks Ixodes ricinus. Cas. Lek. Cesk.88:1179-1180. (In Czech.)","journal-title":"Cas. Lek. Cesk."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2001.0893"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.12.013"},{"key":"e_1_3_2_34_2","first-page":"167","volume":"48","year":"2004","unstructured":"Rudenko, N., M. Golovchenko, V. Cihlarova, and L. Grubhoffer. 2004. Tick-borne encephalitis virus-specific RT-PCR\u2014a rapid test for detection of the pathogen without viral RNA purification. Acta Virol.48:167-171.","journal-title":"Acta Virol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.04.010"},{"key":"e_1_3_2_36_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(99)80069-3"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(02)00168-3"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006313803979"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00812-5"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.13.47.19046-en"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00103-003-0766-3"},{"key":"e_1_3_2_43_2","first-page":"69","volume":"293","year":"2004","unstructured":"Suss, J., C. Schrader, U. Falk, and N. Wohanka. 2004. Tick-borne encephalitis (TBE) in Germany\u2014epidemiological data, development of risk areas and virus prevalence in field-collected ticks and in ticks removed from humans. Int. J. Med. Microbiol.293(Suppl. 37):69-79.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000283"},{"key":"e_1_3_2_45_2","unstructured":"Arch. Biol. Nauk 1939 56"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00391-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00391-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:47:42Z","timestamp":1645638462000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00391-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":44,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1128\/AEM.00391-10"],"URL":"https:\/\/doi.org\/10.1128\/aem.00391-10","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]},"assertion":[{"value":"2010-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}