{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T23:52:23Z","timestamp":1772149943468,"version":"3.50.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2012,7,15]],"date-time":"2012-07-15T00:00:00Z","timestamp":1342310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium DT 104 is the major pathogen for salmonellosis outbreaks in Europe. We tested if the probiotic bacterium\n            <jats:named-content content-type=\"genus-species\">Enterococcus faecium<\/jats:named-content>\n            NCIMB 10415 can prevent or alleviate salmonellosis. Therefore, piglets of the German Landrace breed that were treated with\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 16) as a feed additive and untreated controls (\n            <jats:italic>n<\/jats:italic>\n            = 16) were challenged with\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium 10 days after weaning. The presence of salmonellae in feces and selected organs, as well as the immune response, were investigated. Piglets treated with\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            gained less weight than control piglets (\n            <jats:italic>P<\/jats:italic>\n            = 0.05). The feeding of\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            had no effect on the fecal shedding of salmonellae and resulted in a higher abundance of the pathogen in tonsils of all challenged animals. The specific (anti-\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            IgG) and nonspecific (haptoglobin) humoral immune responses as well as the cellular immune response (T helper cells, cytotoxic T cells, regulatory T cells, \u03b3\u03b4 T cells, and B cells) in the lymph nodes, Peyer's patches of different segments of the intestine (jejunal and ileocecal), the ileal papilla, and in the blood were affected in the course of time after infection (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) but not by the\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            treatment. These results led to the conclusion that\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            may not have beneficial effects on the performance of weaned piglets in the case of\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium infection. Therefore, we suggest a critical discussion and reconsideration of\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            NCIMB 10415 administration as a probiotic for pigs.\n          <\/jats:p>","DOI":"10.1128\/aem.00395-12","type":"journal-article","created":{"date-parts":[[2012,4,28]],"date-time":"2012-04-28T03:53:12Z","timestamp":1335585192000},"page":"4816-4825","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["No Beneficial Effects Evident for Enterococcus faecium NCIMB 10415 in Weaned Pigs Infected with Salmonella enterica Serovar Typhimurium DT104"],"prefix":"10.1128","volume":"78","author":[{"given":"Susanne","family":"Kreuzer","sequence":"first","affiliation":[{"name":"Humboldt-Universit\u00e4t zu Berlin, Breeding Biology and Molecular Genetics, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawel","family":"Janczyk","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Department of Biological Safety, Molecular Diagnostics and Genetics Unit, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"A\u00dfmus","sequence":"additional","affiliation":[{"name":"Humboldt-Universit\u00e4t zu Berlin, Breeding Biology and Molecular Genetics, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael F. G.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Freie Universit\u00e4t Berlin, Institut f\u00fcr Immunologie und Molekularbiologie, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudrun A.","family":"Brockmann","sequence":"additional","affiliation":[{"name":"Humboldt-Universit\u00e4t zu Berlin, Breeding Biology and Molecular Genetics, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"N\u00f6ckler","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Department of Biological Safety, Molecular Diagnostics and Genetics Unit, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01840-06"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.10.005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2010.1496"},{"key":"e_1_3_3_5_2","first-page":"L268\/29","article-title":"Additives for use in animal nutrition. Regulation (EC) no. 1831\/2003","volume":"203","author":"European Parliament and the Council of the European Union","year":"2003","unstructured":"European Parliament and the Council of the European Union . 2003. Additives for use in animal nutrition. Regulation (EC) no. 1831\/2003. Offic. J. Eur. Parliament Council 203: L268\/29.","journal-title":"Offic. J. Eur. Parliament Council"},{"key":"e_1_3_3_6_2","volume-title":"Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria","author":"FAO\/WHO.","year":"2001","unstructured":"FAO\/WHO. 2001. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. FAO\/WHO, Rome, Italy."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)90029-9"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041356"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.06.003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00088"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/650733"},{"key":"e_1_3_3_12_2","first-page":"227","article-title":"Verbesserung der Gesundheit von Ferkeln und Sauen","volume":"5","author":"M\u00e4nner KBK","year":"1991","unstructured":"M\u00e4nnerKBK SchneiderD . 1991. Verbesserung der Gesundheit von Ferkeln und Sauen. Kraftfutter 5: 227\u2013231.","journal-title":"Kraftfutter"},{"key":"e_1_3_3_13_2","first-page":"55","volume-title":"Influence of Enterococcus faecium NCIMB 10415 on performance, small intestinal morphology, gene expression and brush border enzyme activity in piglets","author":"Martin L","year":"2011","unstructured":"MartinL . 2011. Influence of Enterococcus faecium NCIMB 10415 on performance, small intestinal morphology, gene expression and brush border enzyme activity in piglets, p 55. In Proceedings of the International Scientific Conference on Probiotics and Prebiotics IPC2011. Pamida International, Kosice, Slovakia."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.08.010"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4346-4353.2005"},{"key":"e_1_3_3_16_2","first-page":"341","article-title":"Immunomodulatory effect of fibres, probiotics and synbiotics in different life-stages","volume":"25","author":"Romeo J","year":"2010","unstructured":"RomeoJ . 2010. Immunomodulatory effect of fibres, probiotics and synbiotics in different life-stages. Nutr. Hosp. 25: 341\u2013349.","journal-title":"Nutr. Hosp."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2005.02.003"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2004009"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.08.013"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00641.x"},{"key":"e_1_3_3_21_2","first-page":"413","article-title":"The potential of probiotics: a review","volume":"48","author":"Soccol CR","year":"2010","unstructured":"SoccolCR . 2010. The potential of probiotics: a review. Food Technol. Biotechnol. 48: 413\u2013434.","journal-title":"Food Technol. Biotechnol."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01515-08"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2527\/2006.843608x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.d01-705.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0396.2005.00615.x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00395-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00395-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T01:23:58Z","timestamp":1645579438000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00395-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,15]]},"references-count":24,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2012,7,15]]}},"alternative-id":["10.1128\/AEM.00395-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.00395-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,15]]},"assertion":[{"value":"2012-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}