{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:28:53Z","timestamp":1792553333668,"version":"4.5.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,7,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Salicylic acid (SA) and jasmonic acid (JA) pathways are crucial components of plant defense mechanisms; however, their roles in post-harvest preservation remain inadequately explored. Cherry tomatoes are highly susceptible to fungal pathogens, such as\n                      <jats:italic toggle=\"yes\">Alternaria nees<\/jats:italic>\n                      , after harvest, leading to significant post-harvest losses. This study investigates the effects of oligandrin, an elicitin protein derived from\n                      <jats:italic toggle=\"yes\">Pythium oligandrum<\/jats:italic>\n                      , on enhancing the resistance of harvested cherry tomatoes to\n                      <jats:italic toggle=\"yes\">A. nees<\/jats:italic>\n                      . Oligandrin treatment significantly reduced disease symptoms, including black spots and decay, during storage without affecting\n                      <jats:italic toggle=\"yes\">A. nees<\/jats:italic>\n                      spore germination. Treated samples exhibited enhanced activities of defense-related enzymes, including polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and catalase (CAT), compared to controls. Key SA-responsive genes, such as\n                      <jats:italic toggle=\"yes\">NPR1<\/jats:italic>\n                      (Non-expressor of Pathogenesis-Related Genes 1),\n                      <jats:italic toggle=\"yes\">TGA2<\/jats:italic>\n                      (TGACG-Binding Factor 2),\n                      <jats:italic toggle=\"yes\">WRKY70<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">PR1<\/jats:italic>\n                      (Pathogenesis-Related Protein 1), were upregulated within 48 h, indicating activation of the SA signaling pathway. Additionally,\n                      <jats:italic toggle=\"yes\">JAZ1<\/jats:italic>\n                      (Jasmonate ZIM-Domain 1), a negative regulator of JA signaling, was elevated at both 24 and 48 h, while\n                      <jats:italic toggle=\"yes\">MYC2<\/jats:italic>\n                      (Myelocytomatosis 2), a transcription factor involved in the JA pathway, showed significant upregulation, suggesting activation of the JA signaling pathway. These findings demonstrate that oligandrin enhances post-harvest fungal disease resistance by modulating both SA and JA pathways and sustaining elevated defense enzyme activity, offering a promising strategy for improving post-harvest preservation and quality maintenance of cherry tomatoes.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study provides compelling evidence that oligandrin activates both salicylic acid and jasmonic acid signaling pathways in cherry tomatoes, enhancing resistance to\n                        <jats:italic toggle=\"yes\">A. nees<\/jats:italic>\n                        . This dual activation not only deepens our understanding of oligandrin\u2019s mechanisms but also underscores the potential of oligandrin and its producer\n                        <jats:italic toggle=\"yes\">P. oligandrum<\/jats:italic>\n                        as a biocontrol agent for post-harvest preservation. By modulating plant immunity and promoting defense enzyme activity, oligandrin strengthens resistance to fungal diseases, offering a promising strategy for maintaining the quality and extending the shelf life of berry-like crops. Given the successful use of\n                        <jats:italic toggle=\"yes\">P. oligandrum<\/jats:italic>\n                        as a biopesticide in Europe and North America, oligandrin and its producer\n                        <jats:italic toggle=\"yes\">P. oligandrum<\/jats:italic>\n                        present an environmentally friendly alternative to chemical pesticides for post-harvest disease management. This research lays the groundwork for further studies aimed at optimizing the application of\n                        <jats:italic toggle=\"yes\">P. oligandrum<\/jats:italic>\n                        in real-world agricultural settings, establishing it as a reliable, sustainable tool for both agricultural and post-harvest management practices. These findings contribute to advancing biocontrol strategies for safer, more sustainable food production systems.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00421-25","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T09:00:51Z","timestamp":1749805251000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Role of oligandrin in enhancing post-harvest disease resistance in cherry tomato through salicylic acid and jasmonic acid signaling pathways"],"prefix":"10.1128","volume":"91","author":[{"given":"Jia-hao","family":"Sun","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0170z8493","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource 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