{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:42Z","timestamp":1787016642023,"version":"build-2736575974"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2021,6,11]]},"abstract":"<jats:p>Some of the most severe outbreaks of COVID-19 have taken place in places where persons live together, such as nursing homes. Wastewater testing from individual buildings could be used for frequent pooled surveillance of virus from all occupants, including those who are contagious, with or without symptoms.<\/jats:p>","DOI":"10.1128\/aem.00433-21","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T10:35:30Z","timestamp":1618569330000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Development of Wastewater Pooled Surveillance of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Congregate Living Settings"],"prefix":"10.1128","volume":"87","author":[{"given":"Lisa M.","family":"Colosi","sequence":"first","affiliation":[{"name":"Department of Engineering Systems &amp; Environment, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie E.","family":"Barry","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shireen M.","family":"Kotay","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Porter","sequence":"additional","affiliation":[{"name":"Department of Engineering Systems &amp; Environment, University of Virginia, Charlottesville, Virginia, USA"},{"name":"School of Data Science, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda D.","family":"Poulter","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Ratliff","sequence":"additional","affiliation":[{"name":"Facilities Management, Energy and Utilities Division, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Simmons","sequence":"additional","affiliation":[{"name":"Facilities Management, Energy and Utilities Division, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limor I.","family":"Steinberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Derek","family":"Wilson","sequence":"additional","affiliation":[{"name":"Facilities Management, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rena","family":"Morse","sequence":"additional","affiliation":[{"name":"Health Information &amp; Technology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Zmick","sequence":"additional","affiliation":[{"name":"Facilities Management, Energy and Utilities Division, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3533-6351","authenticated-orcid":true,"given":"Amy J.","family":"Mathers","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, University of Virginia Health System, Charlottesville, Virginia, USA"},{"name":"Clinical Microbiology Laboratory, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003189"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2610.201886"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6926e2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6913e1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.05.073"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.7326\/M20-2671"},{"key":"e_1_3_2_8_2","volume-title":"COVID-19 guidance for shared or congregate housing. National Center for Immunization and Respiratory Diseases (NCIRD)","author":"Centers for Disease Control and Prevention","year":"2020","unstructured":"Centers for Disease Control and Prevention. 2020. COVID-19 guidance for shared or congregate housing. National Center for Immunization and Respiratory Diseases (NCIRD), CDC, Atlanta, GA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15006-020-0478-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1495"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58728"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2020.2020"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6927e1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/jgs.16784"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.06.22.20136309"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.09.02.20183830"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0869-5"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2196-x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00614-20"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2020.115942"},{"key":"e_1_3_2_21_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.138875"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.139076"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/traa066"},{"key":"e_1_3_2_25_2","first-page":"27","volume-title":"Wastewater surveillance of the COVID-19 genetic signal in sewersheds recommendations from global experts","author":"The Water Research Foundation","year":"2020","unstructured":"The Water Research Foundation. 2020. Wastewater surveillance of the COVID-19 genetic signal in sewersheds recommendations from global experts, p 27. The Water Research Foundation, Alexandria, VA."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/taaa116"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.05.21.20109181"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2020.104306"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.139960"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2020.116296"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0684-z"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141245"},{"key":"e_1_3_2_33_2","unstructured":"National Center for Immunization and Respiratory Diseases. 2020. Duration of isolation and precautions for adults with COVID-19. https:\/\/stacks.cdc.gov\/view\/cdc\/90858. Accessed 12 January 2021."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coesh.2020.09.003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00562"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00708-20"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.03.022"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.140910"},{"key":"e_1_3_2_39_2","volume-title":"CDC 2019\u2014novel coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel. Centers for Disease Control and Prevention, Food and Drug Administration emergency use authorization.","author":"Centers for Disease Control and Prevention","year":"2020","unstructured":"Centers for Disease Control and Prevention. 2020. CDC 2019\u2014novel coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel. Centers for Disease Control and Prevention, Food and Drug Administration emergency use authorization. Centers for Disease Control and Prevention, Atlanta, GA."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141364"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.198147"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(20)30083-2"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.45357"},{"key":"e_1_3_2_44_2","volume-title":"Guidance for controlling potential risks to workers exposed to class B biosolids health.","author":"Centers for Disease Control and Prevention","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. Guidance for controlling potential risks to workers exposed to class B biosolids health. National Institute for Occupational Safety and Health, Atlanta, GA."},{"key":"e_1_3_2_45_2","volume-title":"Guidance for reducing health risks to workers handling human waste or sewage.","author":"Centers for Disease Control and Prevention","year":"2015","unstructured":"Centers for Disease Control and Prevention. 2015. Guidance for reducing health risks to workers handling human waste or sewage. Health and Human Services, Atlanta, GA."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-017-6258-y"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170199"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.10.012"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","unstructured":"Arnaout R Lee RA Lee GR Callahan C Yen CF Smith KP Arora R Kirby JE. 2020. SARS-CoV2 testing: the limit of detection matters. bioRxiv 10.1101\/2020.06.02.131144.","DOI":"10.1101\/2020.06.02.131144"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/.38.6.2027-2029.2000"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00433-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00433-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:21:10Z","timestamp":1645626070000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00433-21"}},"subtitle":[],"editor":[{"given":"Harold L.","family":"Drake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,11]]},"references-count":49,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2021,6,11]]}},"alternative-id":["10.1128\/AEM.00433-21"],"URL":"https:\/\/doi.org\/10.1128\/aem.00433-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.10.10.20210484","asserted-by":"object"}]},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,11]]},"assertion":[{"value":"2021-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00433-21"}}