{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:02:42Z","timestamp":1791424962354,"version":"4.3.3"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Animal-associated bacterial communities are infected by bacteriophages, although the dynamics of these infections are poorly understood. Transduction by bacteriophages may contribute to transfer of antimicrobial resistance genes, but the relative importance of transduction among other gene transfer mechanisms is unknown. We therefore developed a candidate deterministic mathematical model of the infection dynamics of enteric coliphages in commensal\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            in the large intestine of cattle. We assumed the phages were associated with the intestine and were predominantly temperate. Model simulations demonstrated how, given the bacterial ecology and infection dynamics, most (&gt;90%) commensal enteric\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            bacteria may become lysogens of enteric coliphages during intestinal transit. Using the model and the most liberal assumptions about transduction efficiency and resistance gene frequency, we approximated the upper numerical limits (\u201cworst-case scenario\u201d) of gene transfer through specialized and generalized transduction in\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            by enteric coliphages when the transduced genetic segment is picked at random. The estimates were consistent with a relatively small contribution of transduction to lateral gene spread; for example, generalized transduction delivered the chromosomal resistance gene to up to 8\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            bacteria\/hour within the population of 1.47 \u00d7 10\n            <jats:sup>8<\/jats:sup>\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            bacteria\/liter luminal contents. In comparison, the plasmidic\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            gene carried by \u223c2% of enteric\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            was transferred by conjugation at a rate at least 1.4 \u00d7 10\n            <jats:sup>3<\/jats:sup>\n            times greater than our generalized transduction estimate. The estimated numbers of transductants varied nonlinearly depending on the ecology of bacteria available for phages to infect, that is, on the assumed rates of turnover and replication of enteric\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.00446-14","type":"journal-article","created":{"date-parts":[[2014,5,10]],"date-time":"2014-05-10T02:27:14Z","timestamp":1399688834000},"page":"4350-4362","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":77,"title":["Modeling the Infection Dynamics of Bacteriophages in Enteric Escherichia coli: Estimating the Contribution of Transduction to Antimicrobial Gene Spread"],"prefix":"10.1128","volume":"80","author":[{"given":"Victoriya V.","family":"Volkova","sequence":"first","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Besser","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yrj\u00f6 T.","family":"Gr\u00f6hn","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4161\/bact.1.1.14942"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04143.x"},{"key":"e_1_3_2_4_2","first-page":"275","volume-title":"Molecular genetics of bacteria","author":"Snyder L","year":"2003","unstructured":"SnyderLChampnessW. 2003. Phage \u03bb and lysogeny, p 275\u2013302. In SnyderLChampnessW (ed), Molecular genetics of bacteria, 2nd ed. ASM Press, Washington, DC.","edition":"2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.27.3.519-534.1978"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(08)60785-X"},{"key":"e_1_3_2_7_2","first-page":"231","volume-title":"Molecular genetics of bacteria","author":"Snyder L","year":"2003","unstructured":"SnyderLChampnessW. 2003. Lytic bacteriophages: genetic analysis and use in transduction, p 231\u2013274. In SnyderLChampnessW (ed), Molecular genetics of bacteria, 2nd ed. ASM Press, Washington, DC.","edition":"2"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016900"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.32.1.68-74.1976"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.39.5.1046-1053.1980"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0036"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09199"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.20.6220-6223.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-9-2039"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1079\/9781845939847.0006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1000748505550"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1637-1640.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.11.032"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01145-07"},{"key":"e_1_3_2_20_2","first-page":"271","article-title":"Diversity and dynamics of bacteriophages in horse feces","volume":"76","author":"Kulikova EE","year":"2007","unstructured":"KulikovaEEIsaevaASRotkinaASManykinAALetarovAV. 2007. Diversity and dynamics of bacteriophages in horse feces. Mikrobiologiia 76:271\u2013278. (In Russian.)","journal-title":"Mikrobiologiia"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504062102"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.7.1873-1879.2002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02173-4"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036738"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/0272-4332.204048"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/38.1.51"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.39.2.676-685.1983"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0235"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02100-08"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040193"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00020-10"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.7.2299-2303.1993"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0732"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02788-10"},{"key":"e_1_3_2_35_2","first-page":"239","article-title":"A comparison of three methods for selecting values of input variables in the analysis of output from a computer code","volume":"21","author":"Mckay MD","year":"1979","unstructured":"MckayMDBeckmanRJConoverWJ. 1979. A comparison of three methods for selecting values of input variables in the analysis of output from a computer code. Technometrics 21:239\u2013245.","journal-title":"Technometrics"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02435-08"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.11.5557-5564.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.134668"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-004-5624-3"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.27.3.535-550.1978"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.994-997.1996"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6609"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030119"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.30"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.27.100173.001225"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.5027-5029.1998"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.5.1301-1305.1991"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.89.3.680-686.1965"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00446-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00446-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T02:46:32Z","timestamp":1645584392000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00446-14"}},"subtitle":[],"editor":[{"given":"D. W.","family":"Schaffner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,7,15]]},"references-count":47,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2014,7,15]]}},"alternative-id":["10.1128\/AEM.00446-14"],"URL":"https:\/\/doi.org\/10.1128\/aem.00446-14","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,15]]},"assertion":[{"value":"2014-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}