{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:48:29Z","timestamp":1758862109734},"reference-count":21,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2007,8,15]],"date-time":"2007-08-15T00:00:00Z","timestamp":1187136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Aculeacin A acylase from\n            <jats:italic>Actinoplanes utahensis<\/jats:italic>\n            produced by\n            <jats:italic>Streptomyces lividans<\/jats:italic>\n            revealed acylase activities that are able to hydrolyze penicillin V and several natural aliphatic penicillins. Penicillin K was the best substrate, showing a catalytic efficiency of 34.79 mM\n            <jats:sup>\u22121<\/jats:sup>\n            s\n            <jats:sup>\u22121<\/jats:sup>\n            . Furthermore, aculeacin A acylase was highly thermostable, with a midpoint transition temperature of 81.5\u00b0C.\n          <\/jats:p>","DOI":"10.1128\/aem.00452-07","type":"journal-article","created":{"date-parts":[[2007,6,23]],"date-time":"2007-06-23T01:23:12Z","timestamp":1182561792000},"page":"5378-5381","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Newly Discovered Penicillin Acylase Activity of Aculeacin A Acylase from\n            <i>Actinoplanes utahensis<\/i>"],"prefix":"10.1128","volume":"73","author":[{"given":"Jesu\u0301s","family":"Torres-Bacete","sequence":"first","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Hormigo","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maribel","family":"Stuart","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Arroyo","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Torres","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a P.","family":"Castillo\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Acebal","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose\u0301 L.","family":"Garci\u0301a","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a Molecular, Centro de Investigaciones Biolo\u0301gicas, CSIC, C\/ Ramiro de Maeztu 9, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"de la Mata","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica y Biologi\u0301a Molecular I, Facultad de Biologi\u0301a, Universidad Complutense, C\/ Jose\u0301 Antonio Nova\u0301is 2, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-002-1113-6"},{"key":"e_1_3_2_3_2","first-page":"130","year":"2005","unstructured":"Arroyo, M., I. de la Mata, D. 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