{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:13:42Z","timestamp":1777616022729,"version":"3.51.4"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,6,15]],"date-time":"2014-06-15T00:00:00Z","timestamp":1402790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            can cause severe food-borne disease (listeriosis). Numerous outbreaks have involved three serotype 4b epidemic clones (ECs): ECI, ECII, and ECIa. However, little is known about the population structure of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            serotype 4b from sporadic listeriosis in the United States, even though most cases of human listeriosis are in fact sporadic. Here we analyzed 136 serotype 4b isolates from sporadic cases in the United States, 2003 to 2008, utilizing multiple tools including multilocus genotyping, pulsed-field gel electrophoresis, and sequence analysis of the\n            <jats:italic>inlAB<\/jats:italic>\n            locus. ECI, ECII, and ECIa were frequently encountered (32, 17, and 7%, respectively). However, annually 30 to 68% of isolates were outside these ECs, and several novel clonal groups were identified. An estimated 33 and 17% of the isolates, mostly among the ECs, were resistant to cadmium and arsenic, respectively, but resistance to benzalkonium chloride was uncommon (3%) among the sporadic isolates. The frequency of clonal groups fluctuated within the 6-year study period, without consistent trends. However, on several occasions, temporal clusters of isolates with indistinguishable genotypes were detected, suggesting the possibility of hidden multistate outbreaks. Our analysis suggests a complex population structure of serotype 4b\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            from sporadic disease, with important contributions by ECs and several novel clonal groups. Continuous monitoring will be needed to assess long-term trends in clonality patterns and population structure of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            from sporadic listeriosis.\n          <\/jats:p>","DOI":"10.1128\/aem.00454-14","type":"journal-article","created":{"date-parts":[[2014,4,5]],"date-time":"2014-04-05T03:06:27Z","timestamp":1396667187000},"page":"3632-3644","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Population Structure of Listeria monocytogenes Serotype 4b Isolates from Sporadic Human Listeriosis Cases in the United States from 2003 to 2008"],"prefix":"10.1128","volume":"80","author":[{"given":"Sangmi","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd J.","family":"Ward","sequence":"additional","affiliation":[{"name":"Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis M.","family":"Graves","sequence":"additional","affiliation":[{"name":"Enteric Diseases Laboratory Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl L.","family":"Tarr","sequence":"additional","affiliation":[{"name":"Enteric Diseases Laboratory Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin M.","family":"Siletzky","sequence":"additional","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Kathariou","sequence":"additional","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1201\/9781420015188.ch4","volume-title":"Listeria, listeriosis, and food safety","author":"Painter J","year":"2007","unstructured":"PainterJSlutskerL. 2007. Listeriosis in humans, p 85\u2013110. In RyserETMarthEH (ed), Listeria, listeriosis, and food safety, 3rd ed. CRC Press, Boca Raton, FL.","edition":"3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.09-1101p1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.01.005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4611-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.07.008"},{"key":"e_1_3_2_7_2","first-page":"681","volume-title":"Listeria, listeriosis, and food safety","author":"Kornacki JL","year":"2007","unstructured":"KornackiJLGurtlerJB. 2007. Incidence and control of Listeria in food processing facilities, p 681\u2013766. In RyserETMarthEH (ed), Listeria, listeriosis, and food safety, 3rd ed. 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Update: multistate outbreak of listeriosis\u2014United States, 1998-1999. MMWR Morb. Mortal. Wkly. Rep. 47:1117\u20131118.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.3.574"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid\/1706.101778"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-5-1095"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.7.2707-2716.1997"},{"key":"e_1_3_2_48_2","first-page":"1097","article-title":"Outbreak of Listeria monocytogenes infections associated with pasteurized milk from a local dairy\u2014Massachusetts, 2007","volume":"57","author":"Centers for Disease Control and Prevention (CDC)","year":"2008","unstructured":"Centers for Disease Control and Prevention (CDC). 2008. Outbreak of Listeria monocytogenes infections associated with pasteurized milk from a local dairy\u2014Massachusetts, 2007. MMWR Morb. Mortal. Wkly. Rep. 57:1097\u20131100.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00454-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00454-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:56:47Z","timestamp":1645646207000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00454-14"}},"subtitle":[],"editor":[{"given":"M. W.","family":"Griffiths","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,6,15]]},"references-count":47,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,6,15]]}},"alternative-id":["10.1128\/AEM.00454-14"],"URL":"https:\/\/doi.org\/10.1128\/aem.00454-14","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,15]]},"assertion":[{"value":"2014-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}