{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:58:14Z","timestamp":1781765894281,"version":"3.54.5"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2008,8,15]],"date-time":"2008-08-15T00:00:00Z","timestamp":1218758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lyme borreliosis, the most commonly reported vector-borne disease in North America, is caused by the spirochete\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            . Given the extensive genetic polymorphism of\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>burgdorferi<\/jats:italic>\n            , elucidation of the population genetic structure of the bacterium in clinical samples may be relevant for understanding disease pathogenesis and may have applicability for the development of diagnostic tests and vaccine preparations. In this investigation, the genetic polymorphism of the 16S-23S rRNA (\n            <jats:italic>rrs<\/jats:italic>\n            -\n            <jats:italic>rrlA<\/jats:italic>\n            ) intergenic spacer and\n            <jats:italic>ospC<\/jats:italic>\n            was investigated at the sequence level in 127 clinical isolates obtained from patients with early Lyme borreliosis evaluated in suburban New York City. Sixteen distinct\n            <jats:italic>rrs<\/jats:italic>\n            -\n            <jats:italic>rrlA<\/jats:italic>\n            and 16 distinct\n            <jats:italic>ospC<\/jats:italic>\n            alleles were identified, representing virtually all of the genotypes previously found in questing\n            <jats:italic>Ixodes scapularis<\/jats:italic>\n            nymphs in this region. In addition, a new\n            <jats:italic>ospC<\/jats:italic>\n            group was identified in a single patient. The strong linkage observed between the chromosome-located\n            <jats:italic>rrs<\/jats:italic>\n            -\n            <jats:italic>rrlA<\/jats:italic>\n            and plasmid-borne\n            <jats:italic>ospC<\/jats:italic>\n            genes suggests a clonal structure of\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>burgdorferi<\/jats:italic>\n            in these isolates, despite evidence of recombination at\n            <jats:italic>ospC<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.00479-08","type":"journal-article","created":{"date-parts":[[2008,6,7]],"date-time":"2008-06-07T01:11:00Z","timestamp":1212801060000},"page":"5008-5014","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["<i>Borrelia burgdorferi<\/i>\n            Sensu Stricto Is Clonal in Patients with Early Lyme Borreliosis"],"prefix":"10.1128","volume":"74","author":[{"given":"Kla\u0301ra","family":"Hanincova\u0301","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionysios","family":"Liveris","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Sandigursky","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary P.","family":"Wormser","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla, New York 10595"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 10595"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.02.008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.028738"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26944-0"},{"key":"e_1_3_2_5_2","first-page":"573","volume":"56","year":"2007","unstructured":"Centers for Disease Control and Prevention. 2007. 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