{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:40:39Z","timestamp":1790145639599,"version":"4.0.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2010,9,15]],"date-time":"2010-09-15T00:00:00Z","timestamp":1284508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nitrogen is often a limiting nutrient in natural habitats. Therefore, cyanobacteria have developed multiple responses, which are controlled by transcription factor NtcA and the PII-signaling protein, to adapt to nitrogen deficiency. Transcriptional analyses of\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    sp. strain PCC 6803 under nitrogen-deficient conditions revealed a highly induced gene (\n                    <jats:italic>sll0783<\/jats:italic>\n                    ) which is annotated as encoding a conserved protein with an unknown function. This gene is part of a cluster of seven genes and has potential NtcA-binding sites in the upstream region. Homologues of this cluster occur in some unicellular, nondiazotrophic cyanobacteria and in several\n                    <jats:italic>Alpha<\/jats:italic>\n                    ,\n                    <jats:italic>Beta<\/jats:italic>\n                    -, and\n                    <jats:italic>Gammaproteobacteria<\/jats:italic>\n                    , as well as in some Gram-positive bacteria. Most of the heterotrophic bacteria harboring this gene cluster are able to fix nitrogen and to produce polyhydroxybutyrate (PHB), whereas of the cyanobacteria, only\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    sp. strain PCC 6803 can accumulate PHB. In this work, a\n                    <jats:italic>Synechocystis<\/jats:italic>\n                    sp. strain PCC 6803\n                    <jats:italic>sll0783<\/jats:italic>\n                    gene knockout mutant is characterized. This mutant is unable to accumulate PHB, a carbon and energy storage compound. In contrast, the levels of the carbon storage compound glycogen and the PHB precursor acetyl coenzyme A were similar to those of the wild type, indicating that the PHB-deficient phenotype does not likely result from a global deficiency in carbon metabolism. A specific deficiency in PHB synthesis was implied by the fact that the mutant exhibits impaired PHB synthase activity during prolonged nitrogen starvation. However, the expression of PHB synthase-encoding genes was not strongly affected in the mutant, suggesting that the impaired PHB synthase activity observed depends on a posttranscriptional process in which the product of\n                    <jats:italic>sll0783<\/jats:italic>\n                    is involved.\n                  <\/jats:p>","DOI":"10.1128\/aem.00484-10","type":"journal-article","created":{"date-parts":[[2010,7,30]],"date-time":"2010-07-30T21:44:46Z","timestamp":1280526286000},"page":"6101-6107","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":114,"title":["Requirement of the Nitrogen Starvation-Induced Protein Sll0783 for Polyhydroxybutyrate Accumulation in\n                    <i>Synechocystis<\/i>\n                    sp. Strain PCC 6803"],"prefix":"10.1128","volume":"76","author":[{"given":"Maximilian","family":"Schlebusch","sequence":"first","affiliation":[{"name":"Interfakulta\u0308res Institut fu\u0308r Mikrobiologie und Infektionsmedizin Tu\u0308bingen, Eberhard-Karls-Universita\u0308t, Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Forchhammer","sequence":"additional","affiliation":[{"name":"Interfakulta\u0308res Institut fu\u0308r Mikrobiologie und Infektionsmedizin Tu\u0308bingen, Eberhard-Karls-Universita\u0308t, Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.8.2582-2591.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-005-0056-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409755"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_2_7_2","unstructured":"Acetyl coenzyme A 1974"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb05866.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00960.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.103341"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg563"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)53055-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-009-0972-9"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050629"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4359-4366.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.2.411-425.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.04.003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/183108a0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_20_2","unstructured":"Molecular cloning: a laboratory manual 1982"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn845"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh454"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.16.5009-5013.1997"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1039\/b616219n"},{"key":"e_1_3_2_25_2","first-page":"357","volume":"12","year":"1996","unstructured":"Page, R. 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