{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T13:58:30Z","timestamp":1782914310002,"version":"3.54.5"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2007,8,15]],"date-time":"2007-08-15T00:00:00Z","timestamp":1187136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Pseudomonas pseudoalcaligenes<\/jats:italic>CECT5344 grows in minimal medium containing cyanide as the sole nitrogen source. Under these conditions, an O<jats:sub>2<\/jats:sub>-dependent respiration highly resistant to cyanide was detected in cell extracts. The structural genes for the cyanide-resistant terminal oxidase,<jats:italic>cioA<\/jats:italic>and<jats:italic>cioB<\/jats:italic>, are clustered and encode the integral membrane proteins that correspond to subunits I and II of classical cytochrome<jats:italic>bd<\/jats:italic>, although the presence of heme<jats:italic>d<\/jats:italic>in the membrane could not be detected by difference spectra. The<jats:italic>cio<\/jats:italic>operon from<jats:italic>P. pseudoalcaligenes<\/jats:italic>presents a singular organization, starting upstream of<jats:italic>cioAB<\/jats:italic>by the coding sequence of a putative ferredoxin-dependent sulfite or nitrite reductase and spanning downstream two additional open reading frames that encode uncharacterized gene products. PCR amplifications of RNA (reverse transcription-PCR) indicated the cyanide-dependent up-regulation and cotranscription along the operon. The targeted disruption of<jats:italic>cioA<\/jats:italic>eliminates both the expression of the cyanide-stimulated respiratory activity and the growth with cyanide as the nitrogen source, which suggests a critical role of this cytochrome<jats:italic>bd<\/jats:italic>-related oxidase in the metabolism of cyanide by<jats:italic>P. pseudoalcaligenes<\/jats:italic>CECT5344.<\/jats:p>","DOI":"10.1128\/aem.00503-07","type":"journal-article","created":{"date-parts":[[2007,6,16]],"date-time":"2007-06-16T11:57:59Z","timestamp":1181995079000},"page":"5118-5124","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Essential Role of Cytochrome<i>bd<\/i>-Related Oxidase in Cyanide Resistance of<i>Pseudomonas pseudoalcaligenes<\/i>CECT5344"],"prefix":"10.1128","volume":"73","author":[{"given":"Alberto","family":"Quesada","sequence":"first","affiliation":[{"name":"Departamento de Bioqui\u0301mica, Biologi\u0301a Molecular y Gene\u0301tica, Facultad de Veterinaria, Universidad de Extremadura, Ca\u0301ceres, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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