{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:34:09Z","timestamp":1791171249855,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Reporter gene fusions are essential tools for the investigation of gene regulation. Such fusions are traditionally generated by transposon mutagenesis and identified by a suitable selection procedure. Alternatively, specific reporter fusions can be generated by cloning of DNA fragments containing promoters or other regulatory elements in reporter plasmids. Here, we describe a novel approach for the rapid generation of reporter gene fusions in single copies at defined positions in bacterial genomes. This technique utilizes the Red recombinase for the homologous recombination of PCR-generated cassettes containing various currently used reporter genes, such as those for \u03b2-galactosidase, luciferase, and green fluorescent protein. The approach allows the generation of transcriptional or translational reporter fusions in a single step without the requirement for recombinant DNA constructs and is applicable to various enterobacterial species. Generation of reporter fusions by Red recombination is rapid, overcomes the current limitations of transposon mutagenesis or reporter plasmids, and offers new options for the study of bacterial gene regulation.<\/jats:p>","DOI":"10.1128\/aem.00509-07","type":"journal-article","created":{"date-parts":[[2007,5,19]],"date-time":"2007-05-19T14:57:56Z","timestamp":1179586676000},"page":"4234-4242","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Rapid Engineering of Bacterial Reporter Gene Fusions by Using Red Recombination"],"prefix":"10.1128","volume":"73","author":[{"given":"Roman G.","family":"Gerlach","sequence":"first","affiliation":[{"name":"Mikrobiologisches Institut, Universita\u0308tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie U.","family":"Ho\u0308lzer","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut, Universita\u0308tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Ja\u0308ckel","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut, Universita\u0308tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hensel","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut, Universita\u0308tsklinikum Erlangen, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18040715.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011547"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00685-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01312.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400326101"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00551-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.17.5040-5047.1995"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7480-7491.2003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1598-1605.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00334.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.05.001"},{"key":"e_1_3_2_14_2","first-page":"195","volume":"356","year":"2007","unstructured":"Los, G. V., and K. Wood. 2007. The HaloTag: a novel technology for cell imaging and protein analysis. Methods Mol. Biol.356:195-208.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_15_2","unstructured":"Genetic analysis of pathogenic bacteria 1996"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.13.7539"},{"key":"e_1_3_2_17_2","unstructured":"A short course in bacteria genetics 1992"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123818"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90095-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)04011-C"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35247-X"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb02191.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00787-3"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/nbt804","volume":"21","year":"2003","unstructured":"Testa, G., Y. Zhang, K. Vintersten, V. Benes, W. W. Pijnappel, I. Chambers, A. J. Smith, A. G. Smith, and A. F. Stewart. 2003. Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles. Nat. Biotechnol.21:443-447.","journal-title":"Nat. Biotechnol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5227-5234.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.509"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261348198"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02394-6"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100127597"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00509-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.00509-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T21:05:17Z","timestamp":1645650317000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.00509-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":28,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1128\/AEM.00509-07"],"URL":"https:\/\/doi.org\/10.1128\/aem.00509-07","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7]]},"assertion":[{"value":"2007-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}