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Previous studies have demonstrated that the indole derivative indole-3-ethanol (also known as tryptophol) controls morphogenesis as a QS molecule in fungi. However, whether this QS signal is involved in the modulation of biological functions in bacteria remains unknown. Here, we report that indole-3-ethanol controls the biological functions and pathogenicity of\n              <jats:italic toggle=\"yes\">Enterobacter cloacae<\/jats:italic>\n              subsp.\n              <jats:italic toggle=\"yes\">cloacae<\/jats:italic>\n              ATCC 13047. The biosynthesis of indole-3-ethanol is performed by YjgB (ECL_RS22935), an alcohol dehydrogenase. Deletion of\n              <jats:italic toggle=\"yes\">yjgB<\/jats:italic>\n              results in impaired biological functions and virulence. Furthermore, we revealed that indole-3-ethanol from\n              <jats:italic toggle=\"yes\">E. cloacae<\/jats:italic>\n              reduces the competitive fitness of\n              <jats:italic toggle=\"yes\">Shigella sonnei<\/jats:italic>\n              by inhibiting its biofilm formation, extracellular polysaccharide synthesis, and virulence. Given that both\n              <jats:italic toggle=\"yes\">E. cloacae<\/jats:italic>\n              and\n              <jats:italic toggle=\"yes\">S. sonnei<\/jats:italic>\n              are common human intestinal microbes, our results highlight the critical roles of indole-3-ethanol in both intraspecies signaling and interspecies communication in bacteria.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Quorum sensing is a cell-cell communication mechanism widely employed by bacteria to control various biological functions and pathogenicity. In this study, we demonstrated that\n              <jats:italic toggle=\"yes\">Enterobacter cloacae<\/jats:italic>\n              employs indole-3-ethanol as a quorum-sensing signal to control biological functions and virulence. We also revealed that indole-3-ethanol from\n              <jats:italic toggle=\"yes\">E. cloacae<\/jats:italic>\n              effectively inhibits biofilm formation and virulence in\n              <jats:italic toggle=\"yes\">Shigella sonnei<\/jats:italic>\n              . Our findings not only suggest the important role of indole-3-ethanol in the regulation of the pathogenicity of\n              <jats:italic toggle=\"yes\">E. cloacae<\/jats:italic>\n              but also provide new insights into the development of indole-3-ethanol as an anti-virulence agent against\n              <jats:italic toggle=\"yes\">S. sonnei<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.00510-25","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T13:00:36Z","timestamp":1747054836000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["A cell-cell communication signal from\n            <i>Enterobacter cloacae<\/i>\n            interfering with the signaling systems and virulence in\n            <i>Shigella 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