{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:00:02Z","timestamp":1790172002141,"version":"4.1.0"},"reference-count":104,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Lead pollution is recognized as a major global environmental problem that poses a serious threat to ecological integrity and environmental sustainability. The current study shows the bioremedial potential of Pb-resistant\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. PbS1A2 in\n                      <jats:italic toggle=\"yes\">Labeo rohita<\/jats:italic>\n                      . The fish were divided into three experimental treatments, namely, Control (T\n                      <jats:sub>0<\/jats:sub>\n                      ), Pb only (T\n                      <jats:sub>1<\/jats:sub>\n                      ), and Pb +\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. (T\n                      <jats:sub>2<\/jats:sub>\n                      ). The treatment T\n                      <jats:sub>2<\/jats:sub>\n                      was particularly exposed to a concentration of 1.5 \u00d7 10\n                      <jats:sup>8<\/jats:sup>\n                      CFU\/mL of\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. along with Pb (1 mg L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) for a period of 30 days. The results revealed that\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. PbS1A2 could tolerate Pb concentrations up to 30 mM, along with resistance to other heavy metal ions. Pb exposure significantly increased the activity of all antioxidant enzymes, with glutathione transferase exhibiting the greatest increase (795%), followed by peroxidase (229%), superoxide dismutase (195%), catalase (104%), and ascorbate peroxidase (28%). Furthermore, Fourier transform infrared spectroscopy was employed to identify the functional groups involved in Pb biosorption, while intracellular accumulation was confirmed via energy dispersive X-ray and scanning electron microscopy analyses. At the end of the 1-month trial, fish in the T\n                      <jats:sub>2<\/jats:sub>\n                      group treated with\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. exhibited a significantly higher survival rate, improved growth performance, better body composition, and increased amylase and protease activities than those in the T\n                      <jats:sub>1<\/jats:sub>\n                      group. Furthermore, the T\n                      <jats:sub>2<\/jats:sub>\n                      treatment mitigated the negative impacts of Pb on hematological and biochemical parameters and restored histological damage in the fish organs. The current study suggests that\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp., due to its heavy metal tolerance, could effectively ameliorate Pb bioavailability in aquatic habitats and enhance environmental health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The continuously rising Pb discharge in aquatic ecosystems, particularly in the industrial regions of South Asia, jeopardizes ecological integrity and the health of economically crucial fish such as\n                      <jats:italic toggle=\"yes\">Labeo rohita<\/jats:italic>\n                      , consequently affecting food security. Conventional remediation techniques are often expensive, energy-intensive, and pose ecological risks. This study addresses a significant gap by presenting a thorough bioremediation process encompassing the isolation and characterization of a newly identified multi-metal-resistant\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. (PbS1A2) from industrial wastewater, which facilitates\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      recovery in\n                      <jats:italic toggle=\"yes\">Labeo rohita<\/jats:italic>\n                      contaminated with Pb. Isolated from a contaminated site,\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      sp. have evolved natural metal-resistant mechanisms under environmental stress, resulting in better remediation than lab-engineered options. This is distinguished from studies that solely address the\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      efficiencies of Pb removal. The research offers extensive biological evidence indicating that Pb-induced damage can be effectively mitigated. The results concerning growth performance, hematological assessments, digestive enzyme activities, tissue accumulation, and histopathological evaluations suggest an economical, expandable, and scientifically validated approach to manage Pb pollution in aquatic ecosystems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.00527-26","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:59:31Z","timestamp":1787324371000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Industrial effluent-derived\n                    <i>Acinetobacter<\/i>\n                    sp. PbS1A2 as a bioremediator against lead toxicity in\n                    <i>Labeo rohita<\/i>"],"prefix":"10.1128","volume":"92","author":[{"given":"Saima","family":"Zafar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051zgra59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, Government College University Faisalabad","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Ashraf","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051zgra59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, Government College University Faisalabad","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sehrish","family":"Fatima","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051zgra59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, Government College University Faisalabad","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farkhanda","family":"Asad","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051zgra59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, Government College University Faisalabad","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kafeel","family":"Ahmad","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zynmr34","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Botany, Baba Guru Nanak University","place":["Nankana Sahib, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Tutar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0468j1635","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Biochemistry, Faculty of Medicine, Recep Tayyip Erdogan University","place":["Rize, Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2820-4454","authenticated-orcid":true,"given":"Mudassir","family":"Hassan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zynmr34","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, Baba Guru Nanak University","place":["Nankana Sahib, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2020.110377"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/27658511.2023.2293239"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-024-06081-5"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11270-024-07646-9"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.643972"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1088\/1748-9326\/abbf7c"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.134519"},{"key":"e_1_3_4_9_2","first-page":"405","article-title":"Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic","volume":"11","author":"Selamoglu Talas Z","year":"2012","unstructured":"Selamoglu Talas Z, Pinar Dundar S, Fuat Gulhan M, Orun I, SJIJoFS K. 2012. Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic. Iran J Fish Sci 11:405\u2013414.","journal-title":"Iran J Fish Sci"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2007.07.008"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-007-9146-5"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3354\/dao02714"},{"key":"e_1_3_4_13_2","first-page":"490","article-title":"Effects of propolis on microbiologic and biochemical parameters of rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide","volume":"11","author":"Fuat Gulhan M","year":"2012","unstructured":"Fuat Gulhan M, Duran A, Selamoglu Talas Z, Kakoolaki S, SJIJoFS M. 2012. Effects of propolis on microbiologic and biochemical parameters of rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide. Iran J Fish Sci 11:490\u2013503.","journal-title":"Iran J Fish Sci"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.22092\/ijfs.2018.117890"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.46718\/JBGSR.2021.10.000239"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/10889868.2025.2503454"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2022.129366"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.116572"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.131874"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2019.04.012"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1434921"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2023.140508"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.9734\/ijecc\/2024\/v14i44118"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/10889868.2023.2281450"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2021.1970512"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-022-03484-1"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/09593330.2024.2311084"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2018.03.096"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.15407\/microbiolj83.03.092"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6439-y"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.36899\/JAPS.2021.1.0213"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/w16202994"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.emcon.2023.100204"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42083-8"},{"key":"e_1_3_4_35_2","volume-title":"Microbiology: a laboratory manual","author":"Cappuccino JG","year":"2005","unstructured":"Cappuccino JG, Sherman N. 2005. Microbiology: a laboratory manual. Pearson\/Benjamin Cummings San Francisco"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00244-005-5029-x"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-82-749"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.0014"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50881-X"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3tb20188k"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-016-0225-9"},{"key":"e_1_3_4_42_2","unstructured":"Aryal S. 2021. McFarland standards-principle preparation uses limitations. Microbe Notes. Available from: https:\/\/microbenotes.com\/mcfarland-standards\/. Retrieved 16 Jun 2022."},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01824"},{"key":"e_1_3_4_44_2","volume-title":"Standard methods for the examination of water and wastewater","author":"Association APH","year":"1917","unstructured":"Association APH, Association AWW, Federation WPC, Federation WE. 1917. Standard methods for the examination of water and wastewater. Vol. 3. American Public Health Association."},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.11.038"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1515\/hfsg.1991.45.s1.87"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-09020-8"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-2817-4.50015-9"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.31893\/jabb.20032"},{"key":"e_1_3_4_50_2","first-page":"297","article-title":"Histopathological changes in the liver of a farmed cyprinid fish, Cyprinus carpio, following exposure to nitrate","volume":"37","author":"Iqbal F","year":"2005","unstructured":"Iqbal F, Qureshi IZ, Ali M. 2005. Histopathological changes in the liver of a farmed cyprinid fish, Cyprinus carpio, following exposure to nitrate. Pak J Zool 37:297\u2013300.","journal-title":"Pak J Zool"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1515\/jcim-2018-0063"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-199809010-00016"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.30.4.291"},{"key":"e_1_3_4_54_2","first-page":"270","volume-title":"Methods of enzymatic analysis","author":"Walter H","year":"1984","unstructured":"Walter H. 1984. Proteases and their inhibitors. 2. 15. 2 method with haemoglobin, casein and azocoll as substrate, p 270\u2013277. In Methods of enzymatic analysis"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(85)90186-5"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(87)90080-9"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(89)90156-7"},{"key":"e_1_3_4_58_2","first-page":"477","volume-title":"Starch and its derivates","author":"Robyt J","year":"1968","unstructured":"Robyt J, Whelan W. 1968. The \u03b2-amylases, p 477\u2013497. In Starch and its derivates. Academic press, London."},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/0025-326X(84)90298-4"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40710-020-00436-5"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-020-08696-2"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094642"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1139\/y59-074"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.2478\/aoas-2021-0026"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2023.140495"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-8969-6"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2021.06.382"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.34172\/apb.2020.023"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819382-2.00020-X"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR12.1764"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.01.027"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-024-12545-x"},{"key":"e_1_3_4_73_2","first-page":"8357","article-title":"Protein profiling and antioxidant enzyme activity of cadmium and lead tolerant Kocuria sp. BRI 36","volume":"13","author":"Bhadekar A","year":"2018","unstructured":"Bhadekar A. 2018. Protein profiling and antioxidant enzyme activity of cadmium and lead tolerant Kocuria sp. BRI 36. Int J Appl Eng Res 13:8357\u20138363.","journal-title":"Int J Appl Eng Res"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13762-024-05706-5"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2005.02.020"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2007.05.021"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfa.2006.12.060"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.35495\/ajab.2023.180"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.15666\/aeer\/2202_16231648"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2024.116755"},{"key":"e_1_3_4_81_2","first-page":"1","article-title":"Behavioural responses in effect to chemical stress in fish: A review","volume":"7","author":"Sharma M","year":"2019","unstructured":"Sharma M, Thakur J, Verma S, Sharma P. 2019. Behavioural responses in effect to chemical stress in fish: A review. Int J Fish Aquat Stud 7:1\u20135.","journal-title":"Int J Fish Aquat Stud"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/534769"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxics10120793"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738122"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5841220"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2019.e00103"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10499-020-00630-0"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738948"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eti.2019.100542"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-007-9157-2"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10646-024-02815-2"},{"key":"e_1_3_4_92_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13762-025-06435-z"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2023.113830"},{"key":"e_1_3_4_94_2","first-page":"146","article-title":"Accumulation of cadmium (Cd) and lead (Pb) in tissues of rohu fish (Labeo rohita) collected from the sewage-fed pond of Kolkata","volume":"7","author":"Adhikary J","year":"2019","unstructured":"Adhikary J, Maity A, Das BK, Ghosh S, Pal P. 2019. Accumulation of cadmium (Cd) and lead (Pb) in tissues of rohu fish (Labeo rohita) collected from the sewage-fed pond of Kolkata. J Entomol Zool Stud 7:146\u2013150.","journal-title":"J Entomol Zool Stud"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2024.127523"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-25781-3"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10532-023-10064-z"},{"key":"e_1_3_4_98_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13762-019-02563-5"},{"key":"e_1_3_4_99_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.epm.2024.11.001"},{"key":"e_1_3_4_100_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-016-0819-x"},{"key":"e_1_3_4_101_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1447-2"},{"key":"e_1_3_4_102_2","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.233780"},{"key":"e_1_3_4_103_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12302-024-01047-9"},{"key":"e_1_3_4_104_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737249"},{"key":"e_1_3_4_105_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aqrep.2021.100678"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00527-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.00527-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:05:02Z","timestamp":1790168702000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.00527-26"}},"subtitle":[],"editor":[{"given":"Marina","family":"Lotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":104,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,23]]}},"alternative-id":["10.1128\/aem.00527-26"],"URL":"https:\/\/doi.org\/10.1128\/aem.00527-26","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]},"assertion":[{"value":"2026-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00527-26"}}