{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:59:53Z","timestamp":1791093593103,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In the ubiquitous marine bacterium\n                    <jats:italic>Pseudoalteromonas tunicata<\/jats:italic>\n                    , subpopulations of cells are killed by the production of an autocidal protein, AlpP, during biofilm development. Our data demonstrate an involvement of this process in two parameters, dispersal and phenotypic diversification, which are of importance for the ecology of this organism and for its survival within the environment. Cell death in\n                    <jats:italic>P. tunicata<\/jats:italic>\n                    wild-type biofilms led to a major reproducible dispersal event after 192 h of biofilm development. The dispersal was not observed with a \u0394AlpP mutant strain. Using flow cytometry and the fluorescent dye DiBAC\n                    <jats:sub>4<\/jats:sub>\n                    (3), we also show that\n                    <jats:italic>P. tunicata<\/jats:italic>\n                    wild-type cells that disperse from biofilms have enhanced metabolic activity compared to those cells that disperse from \u0394AlpP mutant biofilms, possibly due to nutrients released from dead cells. Furthermore, we report that there was considerable phenotypic variation among cells dispersing from wild-type biofilms but not from the \u0394AlpP mutant. Wild-type cells that dispersed from biofilms showed significantly increased variations in growth, motility, and biofilm formation, which may be important for successful colonization of new surfaces. These findings suggest for the first time that the autocidal events mediated by an antibacterial protein can confer ecological advantages to the species by generating a metabolically active and phenotypically diverse subpopulation of dispersal cells.\n                  <\/jats:p>","DOI":"10.1128\/aem.00546-06","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T19:53:37Z","timestamp":1154548417000},"page":"5414-5420","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Ecological Advantages of Autolysis during the Development and Dispersal of\n                    <i>Pseudoalteromonas tunicata<\/i>\n                    Biofilms"],"prefix":"10.1128","volume":"72","author":[{"given":"Anne","family":"Mai-Prochnow","sequence":"first","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-innovation, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy S.","family":"Webb","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-innovation, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda C.","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Staffan","family":"Kjelleberg","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-innovation, University of New South Wales, Sydney, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.11.5773-5778.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.3.1667-1669.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04743.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407460101"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2355-2359.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.11.006"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(84)90535-2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.8.2137-2142.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.3.1027-1036.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.4.1865-1873.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1195-1204.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2002.00322.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.372-378.2002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2000.00107.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-2-405"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.14.4486-4491.2004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1139\/m77-249"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00775.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086462"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2004.00618.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-10-2395"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-4-1205"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1999.tb00656.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7418-7425.2004"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.1.290-301.2002"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.8.2783-2788.1996"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.4.1399-1404.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.16.4693-4698.2003"},{"key":"e_1_3_2_31_2","first-page":"757","year":"2003","unstructured":"Kerr, C. 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