{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T09:08:34Z","timestamp":1773738514024,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A total of 905 enterohemorrhagic\n            <jats:italic>Escherichia coli<\/jats:italic>\n            (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.\n          <\/jats:p>","DOI":"10.1128\/aem.00558-09","type":"journal-article","created":{"date-parts":[[2009,7,7]],"date-time":"2009-07-07T01:46:15Z","timestamp":1246931175000},"page":"5719-5726","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic\n            <i>Escherichia coli<\/i>\n            O157:H7 during Passage in Cattle"],"prefix":"10.1128","volume":"75","author":[{"given":"Noriyo","family":"Yoshii","sequence":"first","affiliation":[{"name":"Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitoshi","family":"Ogura","sequence":"additional","affiliation":[{"name":"Division of Microbial Diseases, Department of Infectious Diseases, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Division of Microbial Diseases, Department of Infectious Diseases, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Ajiro","sequence":"additional","affiliation":[{"name":"Division of Livestock Industry, Department of Agriculture, Forestry and Fisheries, Miyagi Prefectural Government, 3-8-1 Honcho, Aoba-ku, Sendai, Miyagi 980-8570, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiya","family":"Sameshima","sequence":"additional","affiliation":[{"name":"Center for Animal Disease Control and Prevention, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muneo","family":"Nakazawa","sequence":"additional","affiliation":[{"name":"Epidemiological Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Kusumoto","sequence":"additional","affiliation":[{"name":"Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taketoshi","family":"Iwata","sequence":"additional","affiliation":[{"name":"Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Akiba","sequence":"additional","affiliation":[{"name":"Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899002198"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08994.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899002204"},{"key":"e_1_3_2_5_2","first-page":"262","volume":"1","year":"1996","unstructured":"Bock, A., and G. 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