{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:14:46Z","timestamp":1790439286303,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Mycoplasma bovis<\/jats:named-content>\n            is the main causal species of bovine mycoplasmal disease and leads to significant economic losses because of its severe symptoms, strong infectivity, and refractoriness. As for mastitis, culling cows with intramammary infections is a general countermeasure to prevent spreading. The conventional antimicrobial susceptibility test for mycoplasma is time-consuming and troublesome, but no quick and easy method for grasping the antimicrobial susceptibility of the causal strain exists at present. Treatment without antimicrobial susceptibility information may be one reason why\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            infection is refractory. Detecting a mutation involved in decreased susceptibility to antimicrobial agents of the causal strain makes it possible to easily select suitable antimicrobials for treatment, and this technique will help improve the cure rate and prevent the overuse of ineffective antimicrobial agents. In this study, we developed a technique to quickly and easily assess antimicrobial susceptibility based on the genetic characteristics of\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            strains in Japan.\n          <\/jats:p>","DOI":"10.1128\/aem.00575-19","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T12:49:17Z","timestamp":1556628557000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Relationship between Antimicrobial Susceptibility and Multilocus Sequence Type of Mycoplasma bovis Isolates and Development of a Method for Rapid Detection of Point Mutations Involved in Decreased Susceptibility to Macrolides, Lincosamides, Tetracyclines, and Spectinomycin"],"prefix":"10.1128","volume":"85","author":[{"given":"Eiji","family":"Hata","sequence":"first","affiliation":[{"name":"Bacterial Pathogenesis Research Unit, Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiro","family":"Harada","sequence":"additional","affiliation":[{"name":"Tokachi Livestock Hygiene Service Center, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"699","article-title":"Outbreak of pneumonia and arthritis in beef calves associated with Mycoplasma bovis and Mycoplasma californicum","volume":"151","author":"Hewicker-Trautwein M","year":"2002","unstructured":"Hewicker-Trautwein M, Feldmann M, Kehler W, Schmidt R, Thiede S, Seeliger F, Wohlsein P, Ball HJ, Buchenau I, Spergser J, Rosengarten R. 2002. Outbreak of pneumonia and arthritis in beef calves associated with Mycoplasma bovis and Mycoplasma californicum. 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Annual report of Animal Quarantine Service 1980\u20132017. Animal Quarantine Service, Narita, Japan. (In Japanese.)"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087672"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.15-0304"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.2169\/naika.102.2823"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00703-09"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.18"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.02003.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.09.008"},{"key":"e_1_3_3_26_2","first-page":"385","article-title":"Experimental infection of the udder of ewes due to Mycoplasma bovis","volume":"38","author":"Bocklisch H","year":"1991","unstructured":"Bocklisch H, Kreusel S, Bry\u015b A, Pf\u00fctzner H. 1991. Experimental infection of the udder of ewes due to Mycoplasma bovis. 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