{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:57:09Z","timestamp":1790873829270,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"23","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic>, the etiologic agent of listeriosis, remains a serious public health concern, with its frequent occurrence in food environments coupled with a high mortality rate. Among the 13 serovars, human listeriosis is mostly associated with the serovar 4b, 1\/2b, and 1\/2a strains. To investigate the diversity of<jats:italic>L. monocytogenes<\/jats:italic>, the intracellular and extracellular proteins of 12 strains were analyzed by two-dimensional gel electrophoresis. These strains had different origins, belonged to different serovars (4b, 1\/2a, and 1\/2b), and presented with different levels of virulence in chicken embryos. The clustering of the strains in two groups based on proteomic patterns is in agreement with the<jats:italic>L. monocytogenes<\/jats:italic>phylogenetic lineages. Statistical analysis did not allow for identification of proteins specific to the isolate origin or the virulence level of the strains, but 26 and 21 protein spots were shown to be significantly overexpressed and underexpressed, respectively, in the six strains of serovar 1\/2a (lineage II) compared to strains of serovar 1\/2b or 4b. Moreover, a penicillin-binding protein was specific for serovar 1\/2b and two protein spots identified as a serine protease were specific to serovar 4b. These protein spots, identified through peptide mass fingerprinting using matrix-assisted laser desorption ionization-time of flight mass spectrometry, were essentially found in the extracellular proteome and may have uses as potential markers for serotyping and risk analysis.<\/jats:p>","DOI":"10.1128\/aem.00594-08","type":"journal-article","created":{"date-parts":[[2008,10,4]],"date-time":"2008-10-04T01:09:42Z","timestamp":1223082582000},"page":"7399-7409","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Comparative Analysis of Extracellular and Intracellular Proteomes of<i>Listeria monocytogenes<\/i>Strains Reveals a Correlation between Protein Expression and Serovar"],"prefix":"10.1128","volume":"74","author":[{"given":"Emilie","family":"Dumas","sequence":"first","affiliation":[{"name":"UR454 Microbiologie Equipe Qualite\u0301 et Se\u0301curite\u0301 des Aliments"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Meunier","sequence":"additional","affiliation":[{"name":"UR1213 Herbivores Equipe Croissance et Me\u0301tabolisme du Muscle"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Berdague\u0301","sequence":"additional","affiliation":[{"name":"UR370 Qualite\u0301 des Produits Animaux Equipe Typicite\u0301 Aromatique et Authentification"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Chambon","sequence":"additional","affiliation":[{"name":"Plate-Forme de Prote\u0301omique, INRA Centre de Clermont-Ferrand\/Theix, F-63122 Saint-Gene\u0300s Champanelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mickae\u0308l","family":"Desvaux","sequence":"additional","affiliation":[{"name":"UR454 Microbiologie Equipe Qualite\u0301 et Se\u0301curite\u0301 des Aliments"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"He\u0301braud","sequence":"additional","affiliation":[{"name":"UR454 Microbiologie Equipe Qualite\u0301 et Se\u0301curite\u0301 des Aliments"},{"name":"Plate-Forme de Prote\u0301omique, INRA Centre de Clermont-Ferrand\/Theix, F-63122 Saint-Gene\u0300s Champanelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04551.x"},{"key":"e_1_3_2_3_2","first-page":"31812","volume":"281","year":"2006","unstructured":"Archambaud, C., M. 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